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Viral protein sequences were retrieved, and potential nonallergenic, nontoxic, and antigenic epitopes were obtained. Next, cytotoxic and helper T\u2010cell (CTL and HTL, respectively) and B\u2010cell (BCL) epitopes were predicted, and those potential epitopes were fused utilizing proper linkers. The in silico cloning and expression processes were implemented using <jats:italic>Escherichia coli<\/jats:italic> K12. The immune responses were prognosticated using the C\u2010ImmSim server. The MEV construct (29.53\u00a0kDa) included four BCL, two CTL, and four HTL epitopes and adjuvant. The MEV traits were pertinent in terms of antigenicity, non\u2010allergenicity, nontoxicity, physicochemical characters, and stability. The MEV candidate was also highly expressed in <jats:italic>E. coli<\/jats:italic> K12. The strong affinity of MEV\u2010TLR3 was confirmed using molecular docking and molecular dynamics simulation analyses. Immune simulation analyses unraveled durable activation and responses of cellular and humoral arms alongside innate immune responses. The designed MEV candidate demonstrated appropriate traits and was promising in the prediction of immune responses against both Mpox and EBOV agents. Further experimental assessments of the MEV are required to verify its efficacy.<\/jats:p>","DOI":"10.1002\/bab.2646","type":"journal-article","created":{"date-parts":[[2024,8,12]],"date-time":"2024-08-12T02:23:40Z","timestamp":1723429420000},"page":"58-74","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A novel multi\u2010epitope peptide vaccine targeting immunogenic antigens of Ebola and monkeypox viruses with potential of immune responses provocation in silico"],"prefix":"10.1002","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9263-8236","authenticated-orcid":false,"given":"Shirin","family":"Mahmoodi","sequence":"first","affiliation":[{"name":"Department of Medical Biotechnology, School of Advanced Technologies in Medicine Fasa University of Medical Sciences  Fasa Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javad 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