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Ag\u2010Secn\u2010NHE was prepared by simple homogenization\u2012ultrasonication technique and validated by using a ultraviolet\u2012visible scan, dynamic light scattering, transmission electron microscopy (TEM), Fourier\u2010transform infrared spectroscopy, and zeta potential. <jats:italic>Saccharomyces cerevisiae<\/jats:italic> (RCMB 002Y001) is the most effective probiotic\u2010producing organism that demonstrates significant effects when combined with Ag\u2010Secn\u2010NHE. Ag\u2010Secn\u2010NHE\u2010based probiotics showed significant antifungal effect compared to free Ag\u2010Secn\u2010NHE, silver nitrate, silver nanoparticles, secnidazole, secnidazole nanoemulsion, and commercial vaginal wash against multidrug\u2010resistant vaginal pathogens. The highest inhibitory effect was achieved with Ag\u2010Secn\u2010NHE\u2010based probiotic against <jats:italic>Candida auris<\/jats:italic>, <jats:italic>Candida albicans<\/jats:italic>, and <jats:italic>Cryptococcus neoformans<\/jats:italic> with minimal inhibitory concentration (MIC) 0.625\u00a0\u00b1\u00a00.002, 0.00625:1.25\u00a0\u00b1\u00a00.012 and 0.00625:1.25\u00a0\u00b1\u00a00.032\u00a0mg\/mL, respectively, in comparison with Ag\u2010Secn\u2010NHE that show MIC at 0.00625:1.25\u00a0\u00b1\u00a00.612, 0.0125:2.5\u00a0\u00b1\u00a00.812, and 0.0125:2.5\u00a0\u00b1\u00a00.112\u00a0mg\/mL (Ag:Secn). Ag\u2010Secn\u2010NHE\u2010based\u2010 probiotic show minimum fungicidal concentration (MFC) at range from 2.5 to 20\u00a0mg\/mL, wherever free Ag\u2010Secn\u2010NHE show MFC range from 5 to &gt;20\u00a0mg\/mL. Additionally, Ag\u2010Secn\u2010NHE\u2010based probiotics have 75% inhibition of biofilm formation against <jats:italic>C. auris<\/jats:italic> and 60% inhibition of biofilm formation against both <jats:italic>Cryptococcus neoformans<\/jats:italic> and <jats:italic>C. albicans<\/jats:italic> in comparison with free Ag\u2010Secn\u2010NHE. Time\u2010kill curves showed that the antifungal effect of Ag\u2010Secn\u2010NHE\u2010based probiotics was fungistatic at 2MIC value after 4\u00a0h and after 16\u00a0h for Ag\u2010Secn\u2010NHE. TEM photographs showed that <jats:italic>C. auris<\/jats:italic> cells treated with Ag\u2010Secn\u2010NHE\u2010based probiotic formula revealed severe deformations and distored ultrastructural changes. furthermore, results indicated that the Gamma radiation up to 15\u00a0kGy increases production of Ag\u2010Secn\u2010NHE in comparison with non\u2010irradiated one.<\/jats:p>","DOI":"10.1002\/bab.2663","type":"journal-article","created":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T06:14:22Z","timestamp":1726467262000},"page":"295-310","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Silver secnidazole nano\u2010hybrid emulsion\u2010based probiotics as a novel antifungal formula against multidrug\u2010resistant vaginal pathogens"],"prefix":"10.1002","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1784-101X","authenticated-orcid":false,"given":"Farag 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