{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T14:52:18Z","timestamp":1789829538969,"version":"4.0.1"},"reference-count":56,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,9,9]],"date-time":"2024-09-09T00:00:00Z","timestamp":1725840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Liver damage and metabolic dysfunctions, the defining features of non\u2010alcoholic fatty liver disease (NAFLD), are marked by inflammation, oxidative stress, and excessive hepatic fat accumulation. The current therapeutic approaches for NAFLD are limited, necessitating exploring novel treatment strategies. Dioxopiperidinamide derivatives, particularly DOPA\u201033, have shown effective anti\u2010inflammatory and antioxidant properties, potentially offering therapeutic benefits against NAFLD. This study investigated the combined potential of vitamin D\n                    <jats:sub>3<\/jats:sub>\n                    (Vit D\n                    <jats:sub>3<\/jats:sub>\n                    ) and DOPA\u201033 in treating NAFLD. The network pharmacology analysis identified key NAFLD targets modulated by Vit D3 and DOPA\u201033, emphasizing their potential mechanisms of action. In NAFLD\u2010induced zebrafish models, Vit D\n                    <jats:sub>3<\/jats:sub>\n                    and DOPA\u201033 significantly reduced hepatic lipid accumulation, oxidative stress, and apoptosis, demonstrating superior efficacy over individual treatments. The treatment also lowered reactive oxygen species (ROS) levels, decreased liver damage, and enhanced antioxidant defense mechanisms. Moreover, behavioral analyses showed improved locomotion and reduced weight gain in treated zebrafish. Biochemical analyses revealed lower triglycerides (TG) and glucose levels with improved oxidative markers. Furthermore, histological analyses indicated reduced hepatic steatosis and inflammation, with decreased expression of lipogenesis\u2010related genes and inflammatory mediators. Finally, high\u2010performance liquid chromatography (HPLC) confirmed a significant reduction in hepatic cholesterol levels, indicating the effectiveness of the combination therapy in addressing key NAFLD\u2010related dyslipidemias. These findings suggest that Vit D\n                    <jats:sub>3<\/jats:sub>\n                    + DOPA\u201033 targets pathways involved in lipid metabolism, inflammation, and oxidative stress by offering a promising therapeutic approach for NAFLD.\n                  <\/jats:p>","DOI":"10.1002\/bab.2666","type":"journal-article","created":{"date-parts":[[2024,9,9]],"date-time":"2024-09-09T23:55:10Z","timestamp":1725926110000},"page":"320-339","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Combined effects of vitamin D3 and dioxopiperidinamide derivative on lipid homeostasis, inflammatory pathways, and redox imbalance in non\u2010alcoholic fatty liver disease in vivo zebrafish model"],"prefix":"10.1002","volume":"72","author":[{"given":"Santhanam Sanjai","family":"Dharshan","sequence":"first","affiliation":[{"name":"Toxicology and Pharmacology Laboratory, Department of Biotechnology, Faculty of Science and Humanities SRM Institute of Science and Technology  Kattankulatur Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karthikeyan","family":"Ramamurthy","sequence":"additional","affiliation":[{"name":"Toxicology and Pharmacology Laboratory, Department of Biotechnology, Faculty of Science and Humanities SRM Institute of Science and Technology  Kattankulatur Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salamuthu","family":"Kaliraj","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Engineering and Technology SRM Institute of Science and Technology  Kattankulathur Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishnan","family":"Manikandan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, SRM College of Pharmacy SRM Institute of Science and Technology  Kattankulathur Tamil Nadu 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Raja","family":"Namasivayam","sequence":"additional","affiliation":[{"name":"Centre for Applied Research, Saveetha School of Engineering Saveetha Institute of Medical and Technical Sciences (SIMATS)  Chennai Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muthu Kumaradoss","family":"Kathiravan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, SRM College of Pharmacy SRM Institute of Science and Technology  Kattankulathur Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesu","family":"Arockiaraj","sequence":"additional","affiliation":[{"name":"Toxicology and Pharmacology Laboratory, Department of Biotechnology, Faculty of Science and Humanities SRM Institute of Science and Technology  Kattankulatur Tamil Nadu 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