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This study aimed to investigate the possible effect of the common food additive monosodium glutamate (MSG) and tannic acid (TA), a phenolic compound, on the key molecular actors responsible for DCM. DCM\u2010related publicly available microarray datasets (GSE120895, GSE17800, and GSE19303) were downloaded from the comprehensive Gene Expression Omnibus (GEO) database, and analyzed to identify differentially expressed genes (DEGs). By integrating DEGs and gene\u2010disease validity curation results, overlapping genes were screened and identified as hub genes. Protein\u2010protein interaction (PPI) network and ontology analysis were performed to make sense of the identified biological data. Finally, mRNA expression changes of identified hub genes in the heart tissues of rats treated with MSG and TA were measured by the qPCR method. Six upregulated (<jats:italic>IGF1, TTN, ACTB, LMNA, EDN1<\/jats:italic>, and <jats:italic>NPPB<\/jats:italic>) DEGs were identified between the DCM and healthy control samples as the hub genes. qPCR results revealed that the mRNA levels of these genes involved in DCM development increased significantly in rat heart tissues exposed to MSG. In contrast, this increase was remarkably alleviated by TA treatment. Our results provide new insights into critical molecular mechanisms that should be focused on in future DCM studies. Moreover, MSG may play a critical role in DCM formation, and TA may be used as a promising therapeutic agent in DCM.<\/jats:p>","DOI":"10.1002\/bab.2670","type":"journal-article","created":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T07:09:33Z","timestamp":1727248173000},"page":"377-387","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Identification of dilated cardiomyopathy\u2010linked key genes by bioinformatics methods and evaluating the impact of tannic acid and monosodium glutamate in rats"],"prefix":"10.1002","volume":"72","author":[{"given":"Habibe","family":"Karadas","sequence":"first","affiliation":[{"name":"Department of Molecular Biology and Genetics, Faculty of Science Atat\u00fcrk University  Erzurum Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilal","family":"Tosun","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics, Faculty 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