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It is endemic in various countries of Asia, Africa, and Europe and is primarily transmitted by <jats:italic>Hyalomma<\/jats:italic> ticks but nosocomial transmission also been reported. Vaccines for CCHF are in early phase of clinical trial; therefore, this work is centered on identification of potential immunogenic peptide as vaccine candidates with application of different immunoinformatics approaches. Eleven conserved (&gt;90%) peptides of CCHFV nucleoprotein were selected for CD8<jats:sup>+<\/jats:sup> T\u2010cell (NetMHCpan 4.1b and NetCTLpan 1.1 server) and CD4<jats:sup>+<\/jats:sup> T\u2010cell (NetMHCIIpan\u20104.0 server and Tepitool) epitope prediction. Three peptides containing multiple CD8<jats:sup>+<\/jats:sup> and CD4<jats:sup>+<\/jats:sup> T\u2010cell and B\u2010cell epitopes were identified on basis of consensus prediction approach. Peptides displayed good antigenicity score of 0.45\u20130.68 and predicted to bind with diverse human leukocyte antigen (HLA) alleles. Molecular docking was performed with epitopes to HLA and HLA\u2010epitopes complex to T\u2010cell receptor (TCR). In most of the cases, docked complex of HLA\u2010epitope and HLA\u2010epitopes\u2010TCR have the binding energy close to respective natural bound peptide complex with HLA and TCR. Molecular dynamic simulation also revealed that HLA\u2010peptide complexes have minimum fluctuation and deviation than HLA\u2010peptide\u2010TCR docked over 50\u00a0ns simulation run. Considering these findings, identified peptides can serve as potential vaccine candidates for CCHFV disease.<\/jats:p>","DOI":"10.1002\/bab.2679","type":"journal-article","created":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T06:34:11Z","timestamp":1728974051000},"page":"498-512","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Computational insights in design of Crimean\u2013Congo hemorrhagic fever virus conserved immunogenic nucleoprotein peptides containing multiple epitopes"],"prefix":"10.1002","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-1209-9421","authenticated-orcid":false,"given":"Neha","family":"Kaushal","sequence":"first","affiliation":[{"name":"Department of Biotechnology Thapar Institute of Engineering and Technology  Patiala Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6581-9346","authenticated-orcid":false,"given":"Manoj","family":"Baranwal","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Thapar Institute of Engineering and Technology  Patiala Punjab India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,10,14]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436\u2010021\u201007114\u2010x"},{"key":"e_1_2_10_3_1","unstructured":"Arboviruses are some of the world's most dangerous mosquito\u2010borne illnesses.Precision Vax;2022. 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