{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T18:57:52Z","timestamp":1766516272347,"version":"3.43.0"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,1,20]],"date-time":"2025-01-20T00:00:00Z","timestamp":1737331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Ulcerative colitis affects the inner lining of the large intestine, causing discomfort, pain, and digestion issues, and sometimes leading to life\u2010threatening complications. With proper medication, symptoms and inflammation can be reduced, improving the condition. In this research, a multiwalled carbon nanotube (MWCN)\u2010modified circular interdigitated electrode (circular\u2010IDE) biosensor was developed to detect the ulcerative colitis biomarker lipocalin\u20102 and measured at 0\u20132\u00a0V. A dual probing strategy with aptamer and antibody on gold nanoparticles was employed for the detection of lipocalin\u20102. Probe immobilization was optimized on MWCN\u2010modified circular\u2010IDE, and saturation of 800\u00a0nM of aptamer on the GNP\u2010antibody facilitated the identification of lipocalin\u20102 at concentrations as low as 1 pg\/mL, with an <jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> value of 0.9716 [<jats:italic>y<\/jats:italic> = 2.1058<jats:italic>x<\/jats:italic> \u2212 2.7351]. Furthermore, lipocalin\u20102 spiking in serum increased the current responses in correlation with the concentrations of lipocalin\u20102, indicating selective identification without interference. In addition, nonimmune antibody and GNP\u2010conjugated complementary aptamer did not increase the current responses, affirming the specific detection of lipocalin\u20102. This MWCN\u2010modified circular\u2010IDE biosensor, utilizing aptamer\u2013antibody interactions, aids in identifying the condition of ulcerative colitis.<\/jats:p>","DOI":"10.1002\/bab.2715","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T02:14:44Z","timestamp":1737425684000},"page":"999-1005","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Lipocalin\u20102 Determination on Multiwalled Carbon Nanotube Integrated Circular Electrodes for Diagnosing Ulcerative Colitis"],"prefix":"10.1002","volume":"72","author":[{"given":"Guangchao","family":"Qiao","sequence":"first","affiliation":[{"name":"Department of Gastrointestinal Surgery The Fifth People's Hospital of Jinan  Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2050-9333","authenticated-orcid":false,"given":"Jijian","family":"Sheng","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery The Fifth People's Hospital of Jinan  Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,20]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"50","article-title":"Research Progress of the Influence of Appendectomy on Ulcerative Colitis","volume":"5","author":"Zhang Z.","year":"2021","journal-title":"Chinese Journal of Inflammatory Bowel Diseases"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.50.4.501"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.36922\/gtm.0403"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.36922\/an.v1i2.71"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.36922\/bh.v1i1.223"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.36922\/arnm.0883"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.36922\/jcbp.0571"},{"issue":"4","key":"e_1_2_5_9_1","first-page":"135","article-title":"A Double\u2010Blind, Randomized Trial on the Effect of a Broad\u2010Spectrum Dietary Supplement on Key Biomarkers of Cellular Aging in Healthy Adults","volume":"2","author":"Lages L. C.","year":"2016","journal-title":"Journal of Clinical and Translational Research"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.18063\/ijps.v8i1.1285"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.36922\/td.v1i2.165"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.36922\/gpd.0658"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.36922\/an.v1i2.116"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.36922\/bh.v1i1.188"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.36922\/gpd.305"},{"issue":"2","key":"e_1_2_5_16_1","first-page":"60","article-title":"Circulating Plasma microRNA\u2010126, \u2010145 and \u2010155 and Their Association With Atherosclerotic Plaque Characteristics","volume":"5","author":"Knoka E.","year":"2019","journal-title":"Journal of Clinical and Translational Research"},{"key":"e_1_2_5_17_1","first-page":"93","article-title":"Factors Associated With Knowledge, Attitudes, and Practices of the General Lebanese Population Toward the Coronavirus Disease","volume":"7","author":"Haddad C.","year":"2019","journal-title":"International Journal of Population Studies"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20212418"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044328"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.20142"},{"key":"e_1_2_5_21_1","first-page":"41","article-title":"Flexible, Wearable and Fully\u2010Printed Smart Patch for pH and Hydration Sensing in Wounds","volume":"8","author":"Iversen M.","year":"2022","journal-title":"International Journal of Bioprinting"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12668-021-00847-0"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.36922\/gtm.0394"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.18063\/ijb.v9i1.647"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtice.2021.04.016"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/bab.1961"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.36922\/msam.0620"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.18063\/msam.v1i1.4"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.36922\/td.v1i1.41"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.36922\/itps.v4i2.70"},{"key":"e_1_2_5_31_1","first-page":"1","article-title":"Aptamer\u2010Based Sandwich Assay and Its Clinical Outlooks for Detecting Lipocalin\u20102 in Hepatocellular Carcinoma (HCC)","volume":"5","author":"Lee K. A.","year":"2015","journal-title":"Scientific Reports"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.36922\/itps.298"}],"container-title":["Biotechnology and Applied Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bab.2715","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T07:20:08Z","timestamp":1754896808000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/bab.2715"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,20]]},"references-count":31,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["10.1002\/bab.2715"],"URL":"https:\/\/doi.org\/10.1002\/bab.2715","archive":["Portico"],"relation":{},"ISSN":["0885-4513","1470-8744"],"issn-type":[{"type":"print","value":"0885-4513"},{"type":"electronic","value":"1470-8744"}],"subject":[],"published":{"date-parts":[[2025,1,20]]},"assertion":[{"value":"2024-05-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}