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The absence of non\u2010effective vaccines and specific treatments underscores the need for advanced diagnostic tools for early detection and management. This study presents a novel biosensor for detecting dengue virus type 4 (DENV\u20104) by combining carbonyldiimidazole nanoflower (CDI\u2010NF) with Mn<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub> on laser\u2010scribed graphene (LSG). Material characterization techniques, including Raman spectroscopy, TEM, XRD, XPS, and FTIR, were employed to confirm the successful integration of Mn<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub> and CDI\u2010NF, resulting in a unique 3D flower\u2010like structure. In order to verify the sensing efficiency, a selective DNA sample captured on LSG\/Mn<jats:sub>3<\/jats:sub>O<jats:sub>4<\/jats:sub>\u2010CDI\u2010NF was investigated for specifc binding with <jats:italic>Aedes aegypti<\/jats:italic> target DNA through selective hybridization and mismatch analysis. Electrochemical impedance studies further confirmed sensitive detection of up to 1\u00a0fM, where the sensitivity was confirmed by large transfer resistance (<jats:italic>R<\/jats:italic><jats:sub>ct<\/jats:sub>) before and after hybridization with a regression coefficient 0.97373. EIS results demonstrated successful surface modifications and the biosensor's specificity in distinguishing between complementary, mismatched, and non\u2010complementary target sequences. The biosensor's ability to differentiate between these sequences highlights its potential for accurate and targeted DENV\u20104 detection, offering a promising avenue for advancing dengue diagnostics.<\/jats:p>","DOI":"10.1002\/bab.2731","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T07:44:18Z","timestamp":1739259858000},"page":"1231-1240","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Mn<sub>3<\/sub>O<sub>4<\/sub> Tetrahedral with Carbonyldiimidazole Nanoflower Deposition on Laser\u2010Scribed Graphene for Selective Bio\u2010Capture"],"prefix":"10.1002","volume":"72","author":[{"given":"Sivainesh Devi","family":"Remesh","sequence":"first","affiliation":[{"name":"Department of Mechanical Engineering Universiti Teknologi PETRONAS  Seri Iskandar Perak Darul Ridzuan Malaysia"},{"name":"Centre of Innovative Nanostructures and Nanodevices (COINN) Universiti Teknologi PETRONAS  Seri Iskandar Perak Darul Ridzuan Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeysree","family":"Chelvaraj","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering Universiti Teknologi PETRONAS  Seri Iskandar Perak Darul Ridzuan Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veeradasan","family":"Perumal","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering Universiti Teknologi PETRONAS  Seri Iskandar Perak Darul Ridzuan Malaysia"},{"name":"Centre of Innovative Nanostructures and Nanodevices (COINN) Universiti Teknologi PETRONAS  Seri Iskandar Perak Darul Ridzuan Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8347-4687","authenticated-orcid":false,"given":"Subash C. 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