{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:01:46Z","timestamp":1787068906404,"version":"build-2736575974"},"reference-count":71,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,5,4]],"date-time":"2025-05-04T00:00:00Z","timestamp":1746316800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Alzheimer's disease (AD) is a progressive neurodegenerative disorder that affects millions of individuals worldwide. Treatment strategies for AD vary depending on cognitive and behavioral symptoms, with cholinergic replacement therapy using acetylcholinesterase (AChE) inhibitors being one of the primary approaches. Recent studies have also identified human carbonic anhydrases (hCAs) as significant therapeutic targets for AD, offering new opportunities for the development of innovative treatments. Carbonic anhydrase inhibitors have been shown to prevent early mitochondrial damage and inhibit H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    production, thereby reducing amyloid plaque formation. Building on the promising potential of hydrazones particularly sulfonyl hydrazones as anticholinesterase agents, we synthesized 12 novel chlorine\u2010substituted sulfonyl hydrazone compounds containing aryl sulfonate ester groups. These compounds were evaluated for their inhibitory effects on AChE, hCA I, and hCA II enzymes. The synthesized compounds exhibited low nanomolar inhibitory activity, with\n                    <jats:italic>\n                      K\n                      <jats:sub>i<\/jats:sub>\n                    <\/jats:italic>\n                    values ranging from 9.58\u00a0\u00b1\u00a02.22 to 104.04\u00a0\u00b1\u00a023.82\u00a0nM for AChE, 9.12\u00a0\u00b1\u00a02.21 to 477.63\u00a0\u00b1\u00a0218.52\u00a0nM for hCA I, and 17.54\u00a0\u00b1\u00a07.74 to 564.62\u00a0\u00b1\u00a0213.98\u00a0nM for hCA II. Notably, compound\n                    <jats:bold>6<\/jats:bold>\n                    showed strong inhibitory activity against hCA I (\n                    <jats:italic>\n                      K\n                      <jats:sub>i<\/jats:sub>\n                    <\/jats:italic>\n                    \u00a0=\u00a09.12\u00a0\u00b1\u00a02.21\u00a0nM; acetazolamide (AZA)\u00a0=\u00a026.54\u00a0\u00b1\u00a03.11\u00a0nM) and hCA II (\n                    <jats:italic>\n                      K\n                      <jats:sub>i<\/jats:sub>\n                    <\/jats:italic>\n                    \u00a0=\u00a017.54\u00a0\u00b1\u00a07.74\u00a0nM; AZA\u00a0=\u00a021.73\u00a0\u00b1\u00a02.42\u00a0nM), whereas compound\n                    <jats:bold>4<\/jats:bold>\n                    exhibited superior AChE inhibition (\n                    <jats:italic>\n                      K\n                      <jats:sub>i<\/jats:sub>\n                    <\/jats:italic>\n                    \u00a0=\u00a09.58\u00a0\u00b1\u00a02.22\u00a0nM; TAC\u00a0=\u00a023.12\u00a0\u00b1\u00a02.05\u00a0nM). The chemical structures of the synthesized compounds were characterized using advanced spectroscopic techniques, including FT\u2010IR,\n                    <jats:sup>1<\/jats:sup>\n                    H\u2010NMR, and\n                    <jats:sup>13<\/jats:sup>\n                    C\u2010NMR spectroscopy.\n                  <\/jats:p>","DOI":"10.1002\/bab.2780","type":"journal-article","created":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T02:40:30Z","timestamp":1746412830000},"page":"1752-1762","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Novel Sulfonylhydrazones With Sulfonate Ester Framework: Promising Dual Inhibitors of AChE and hCAs"],"prefix":"10.1002","volume":"72","author":[{"given":"Cansu","family":"\u00d6zt\u00fcrk","sequence":"first","affiliation":[{"name":"Faculty of Science, Department of Chemistry Atat\u00fcrk University  Erzurum Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neslihan","family":"Balci","sequence":"additional","affiliation":[{"name":"Siran Dursun Keles Vocational School of Health Services G\u00fcm\u00fcshane University  G\u00fcm\u00fcshane Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osman Nuri","family":"Aslan","sequence":"additional","affiliation":[{"name":"East Anatolian High Technology Application and Research Center Atat\u00fcrk University  Erzurum Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4656-8254","authenticated-orcid":false,"given":"Erbay","family":"Kalay","sequence":"additional","affiliation":[{"name":"Kars Vocational School, Department of Material and Material Processing Technologies Kafkas University  Kars Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmr.3004"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2023.2240889"},{"issue":"2","key":"e_1_2_8_4_1","first-page":"1479","article-title":"Cholinesterase Inhibitors as Alzheimer's Therapeutics","volume":"20","author":"Sharma K.","year":"2019","journal-title":"Molecular Medicine Reports"},{"key":"e_1_2_8_5_1","volume-title":"Physiology, Acetylcholine","author":"Sam 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