{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T21:15:02Z","timestamp":1788383702726,"version":"build-2803163510"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T00:00:00Z","timestamp":1750896000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100011700","name":"National Medicinal Plants Board, Ministry of AYUSH, Government of India","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Osteoporosis is a widespread condition, particularly affecting women and high\u2010aged groups, with limited allopathic treatment options leading to adverse effects. The interest in natural remedies for osteoporosis is growing, and plant\u2010based compounds are being explored for their potential to promote bone regeneration.\n                    <jats:italic>Vachellia nilotica<\/jats:italic>\n                    , known for its various medicinal properties, has shown promise in traditional medicine but lacks scientific evidence for its role in osteoporosis treatment. This study focused on isolating from\n                    <jats:italic>V. nilotica<\/jats:italic>\n                    leaves and characterizing a compound, methyl 3,4,5\u2010trihydroxybenzoate (methyl gallate), and its action upon osteoblastic cell lines to deal with osteoporotic disease. Methyl gallate is a phenolic compound. Methyl gallate is a substance that is used for many functions in the body; for example, it is used as an anti\u2010inflammatory, an antioxidant, a neuroprotector, a hepato\u2010protector, and an immunomodulator, as well as leading a range of research and development activities. The compound exhibited no cytotoxic effects at low concentrations on the MG 63 cell line, indicating its safety. It also demonstrated significant antioxidant activity, with the ability to scavenge radicals and reduce oxidative stress. Moreover, the isolated compound showed a stimulating effect on alkaline phosphatase activity, a marker of early differentiation of osteoblasts, thereby decreasing the activity of osteoclasts and thus leading to increased mineralization. The above results suggest the compound's potential to promote bone regeneration and healing. Overall, this study provides valuable insights into the compound's osteogenic differentiation properties from\n                    <jats:italic>V. nilotica leaves<\/jats:italic>\n                    and their potential as a natural remedy for osteoporosis.\n                  <\/jats:p>","DOI":"10.1002\/bab.2784","type":"journal-article","created":{"date-parts":[[2025,6,26]],"date-time":"2025-06-26T03:29:08Z","timestamp":1750908548000},"page":"1771-1780","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Methyl Gallate: A Potent Bioactive Compound Promoting Osteogenic Differentiation by Regulating Runx2 and Cbfa1 in Osteoblastic Cell Lines"],"prefix":"10.1002","volume":"72","author":[{"given":"Neha","family":"Verma","sequence":"first","affiliation":[{"name":"Department of Life Sciences, Sharda School of Bio\u2010Science &amp; Technology Sharda University, Knowledge Park\u2010III Greater Noida Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bimalkumar","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Chemistry, School of Science Gujarat University Ahmedabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhawna","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Sharda School of Bio\u2010Science &amp; Technology Sharda University, Knowledge Park\u2010III Greater Noida Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shilpa","family":"Kumari","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Sharda School of Bio\u2010Science &amp; Technology Sharda University, Knowledge Park\u2010III Greater Noida Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Showket","family":"Hussain","sequence":"additional","affiliation":[{"name":"Division of Molecular Biology, National Institute of Cancer Prevention and Research ICMR Noida Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitesh D.","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Chemistry, School of Science Gujarat University Ahmedabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6043-3786","authenticated-orcid":false,"given":"Kanu","family":"Priya","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, Sharda School of Bio\u2010Science &amp; Technology Sharda University, Knowledge Park\u2010III Greater Noida Uttar Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,26]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1297\u2010319X(10)70004\u2010X"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.5152\/eurjrheum.2016.048"},{"issue":"4","key":"e_1_2_11_4_1","first-page":"365","article-title":"Drugs for the Management of Osteoporosis: A Review","volume":"51","author":"Khajuria D. K.","year":"2011","journal-title":"Revista Brasileira De Reumatologia"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40199\u2010017\u20100167\u2010z"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2010.01.057"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1515\/jcim\u20102021\u20100112"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2009.10.013"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2011.08.020"},{"issue":"1","key":"e_1_2_11_10_1","first-page":"1","article-title":"Acute and Chronic Anti\u2010Inflammatory Effects of the Aqueous Extract of Acacia nilotica (L.) Del.(Fabaceae) Pods","volume":"1","author":"Sokeng S.","year":"2013","journal-title":"Academia Journal of Medicinal Plants"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1996.0292"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.00757"},{"issue":"4","key":"e_1_2_11_13_1","first-page":"32","article-title":"The Potential Role of Medicinal Plants in Bone Regeneration","volume":"25","author":"Barbalho S. M.","year":"2019","journal-title":"Alternative Therapies in Health and Medicine"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18030581"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3109\/15368378.2016.1138124"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2018.8628"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.4162\/nrp.2017.11.3.190"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2469"},{"issue":"12","key":"e_1_2_11_19_1","first-page":"2527","article-title":"Potential Mechanisms Underlying the Runx2 Induced Osteogenesis of Bone Marrow Mesenchymal Stem Cells","volume":"7","author":"Xu J.","year":"2015","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(19990401)73:1<114::AID-JCB13>3.0.CO;2-M"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2019.111652"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.4196\/kjpp.2014.18.4.347"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2803"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/7203584"}],"container-title":["Biotechnology and Applied Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bab.2784","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T14:01:41Z","timestamp":1765288901000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/bab.2784"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,26]]},"references-count":23,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/bab.2784"],"URL":"https:\/\/doi.org\/10.1002\/bab.2784","archive":["Portico"],"relation":{},"ISSN":["0885-4513","1470-8744"],"issn-type":[{"value":"0885-4513","type":"print"},{"value":"1470-8744","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,26]]},"assertion":[{"value":"2024-11-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}