{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T14:06:45Z","timestamp":1765289205478,"version":"3.46.0"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T00:00:00Z","timestamp":1748390400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Ulcerative colitis (UC) is a long\u2010term inflammatory condition impacting the bowel with an unclear cause. It is categorized as a refractory condition due to the limited efficacy and adverse effects of existing treatments. Dihydroartemisinin (DHA), a semi\u2010synthetic derivative and primary active metabolite of artemisinin, exhibits anti\u2010inflammatory and antioxidant properties. To assess the therapeutic effects of DHA on UC and elucidate its possible mechanisms of action. A dextran sulfate sodium (DSS)\u2010induced UC mouse model (2.5% DSS for 30 days) was used to evaluate the therapeutic effects of DHA (20\u00a0mg\/kg\/day) through assessment of disease activity, colon damage, and inflammation. Key targets were identified using network pharmacology, followed by pathway analysis (GO and Kyoto Encyclopedia of Genes and Genomes [KEGG]), molecular docking, and western blotting to validate interactions and signaling modulation. DHA treatment significantly improved disease activity index (DAI) scores, reduced colon shortening, and ameliorated histopathological injury in the DSS\u2010induced UC mouse model. Seven core targets of DHA were identified: EGFR, MMP9, PTGS2, MMP2, mitogen\u2010activated protein kinase 3 (MAPK3), MAPK1, and ERBB2. Enrichment analyses revealed critical mechanisms and pathways implicated in its therapeutic effects. Molecular docking demonstrated robust binding between DHA and its targets, whereas western blot analysis confirmed that DHA mitigated UC via modulation of the MAPK inflammatory signaling pathway. The research highlights DHA's therapeutic potential in UC treatment by identifying its core targets and mechanisms of action, paving the way for future research and drug development in managing UC.<\/jats:p>","DOI":"10.1002\/bab.2793","type":"journal-article","created":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T06:54:34Z","timestamp":1748415274000},"page":"1867-1882","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dihydroartemisinin Alleviates Ulcerative Colitis via Target Identification and Pathway Modulation: A Cyberpharmacology Approach"],"prefix":"10.1002","volume":"72","author":[{"given":"Yu","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Shanxi Provincial People's Hospital Affiliated to Shanxi Medical University  Taiyuan Shanxi China"},{"name":"The Fifth Clinical Medical College of Shanxi Medical University  Taiyuan Shanxi 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