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Unlike heterogeneous immunoassays, which require time\u2010consuming separation steps or expensive automated systems, homogeneous immunoassays, wherein all reagents are freely suspended in bulk solution, can be simple and fast without costly instrumentation. The key component of this assay system, an estradiol\u2013reporter enzyme conjugate, was prepared by covalently binding \u03b2\u2010estradiol\u20106\u2010(O\u2010carboxymethyl)oxime to glucose\u20106\u2010phosphate dehydrogenase (G6PDH) by an <jats:italic>N<\/jats:italic>\u2010hydroxysuccinimide\u2010enhanced, carbodiimide\u2010mediated coupling reaction. The estradiol\u2013G6PDH activity can be repressed up to 46% upon anti\u2010estradiol antibody binding. The lower detection limit of the assay is 1 nM estradiol in aqueous solution, and the standard curve is linear on logit\u2010log scale\u2010up to 6.7 \u00b5M estradiol. A detection limit of 11.5 nM in estradiol\u2010spiked human serum samples suggests the feasibility of applying this assay to monitor estradiol levels for the prediction and prevention of ovarian hyperstimulation syndrome.<\/jats:p>","DOI":"10.1002\/bab.5","type":"journal-article","created":{"date-parts":[[2011,4,4]],"date-time":"2011-04-04T08:34:32Z","timestamp":1301906072000},"page":"75-82","source":"Crossref","is-referenced-by-count":12,"title":["A convenient homogeneous enzyme immunoassay for estradiol detection"],"prefix":"10.1002","volume":"58","author":[{"given":"May L.","family":"Chiu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina T.\u2010C.","family":"Tseng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harold G.","family":"Monbouquette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,3,29]]},"reference":[{"key":"e_1_2_8_2_2","first-page":"6","article-title":"Fertility, family planning, and women's health: New data from the 1995 National Survey of Family Growth. 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