{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T04:24:47Z","timestamp":1791779087776,"version":"4.3.4"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T00:00:00Z","timestamp":1748563200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T00:00:00Z","timestamp":1748563200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Helicobacter pylori<\/jats:italic>\n                    \u2010induced inflammatory microenvironment plays a pivotal role in the development of gastric cancer (GC). This study investigates whether matrix metallopeptidase 9 (MMP9) mediates\n                    <jats:italic>H. pylori<\/jats:italic>\n                    \u2010induced effects in GC progression. GC patients were stratified on the basis of\n                    <jats:italic>H. pylori<\/jats:italic>\n                    infection status. MMP9 levels were measured using enzyme\u2010linked immunosorbent assay (ELISA), immunohistochemistry, and RT\u2010PCR. AGS and MKN45 GC cell lines were transfected with MMP9\u2010specific siRNA and cocultured with\n                    <jats:italic>H. pylori<\/jats:italic>\n                    . Cell viability, proliferation, and migration were assessed using 3\u2010[4,5\u2010dimethylthiazol\u20102\u2010yl]\u20102,5 diphenyl tetrazolium bromide assay, colony formation assay, and transwell migration assay, respectively. Expression of pro\u2010inflammatory cytokines in cells and their secretion into coculture supernatants were analyzed by Western blotting and ELISA. MMP9 levels were significantly elevated in the serum of\n                    <jats:italic>H. pylori<\/jats:italic>\n                    \u2010positive GC patients compared to\n                    <jats:italic>H. pylori<\/jats:italic>\n                    \u2010negative patients. Correspondingly, increased MMP9 mRNA and protein expression were detected in\n                    <jats:italic>H. pylori<\/jats:italic>\n                    \u2010positive GC tissues. Serum levels and tissue mRNA expression of interleukin (IL)\u20101\u03b2, tumor necrosis factor (TNF)\u2010\u03b1, and IL\u20108 positively correlated with MMP9 expression. In vitro,\n                    <jats:italic>H. pylori<\/jats:italic>\n                    infection enhanced MMP9 expression and promoted the secretion of IL\u20101\u03b2, TNF\u2010\u03b1, and IL\u20108 in AGS and MKN45 cells.\n                    <jats:italic>H. pylori<\/jats:italic>\n                    stimulation also increased the survival, proliferation, and migration of GC cells, which were significantly attenuated by MMP9 knockdown. MMP9 mediates\n                    <jats:italic>H. pylori<\/jats:italic>\n                    \u2010induced inflammatory responses that contribute to the GC progression. Targeting MMP9 may represent a therapeutic strategy to counteract\n                    <jats:italic>H. pylori<\/jats:italic>\n                    \u2010driven GC development.\n                  <\/jats:p>","DOI":"10.1002\/bab.70000","type":"journal-article","created":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T03:15:19Z","timestamp":1748574919000},"page":"5-13","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Matrix Metalloproteinases 9 Contributes to Inflammatory Responses in\n                    <i>Helicobacter pylori<\/i>\n                    \u2010Positive Gastric Cancer"],"prefix":"10.1002","volume":"73","author":[{"given":"Wen\u2010Jie","family":"Kong","sequence":"first","affiliation":[{"name":"Department of Gastroenterology People's Hospital of Xinjiang Uygur Autonomous Region  Urumqi Xinjiang Uygur Autonomous Region China"},{"name":"Xinjiang Clinical Research Center for Digestive Diseases  Urumqi Xinjiang Uygur Autonomous Region China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei\u2010Dong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology People's Hospital of Xinjiang Uygur Autonomous Region  Urumqi Xinjiang Uygur Autonomous Region China"},{"name":"Xinjiang Clinical Research Center for Digestive Diseases  Urumqi Xinjiang Uygur Autonomous Region China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology People's Hospital of Xinjiang Uygur Autonomous Region  Urumqi Xinjiang Uygur Autonomous Region China"},{"name":"Xinjiang Clinical Research Center for Digestive Diseases  Urumqi Xinjiang Uygur Autonomous Region China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Jia","family":"Hui","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology People's Hospital of Xinjiang Uygur Autonomous Region  Urumqi Xinjiang Uygur Autonomous Region China"},{"name":"Xinjiang Clinical Research Center for Digestive Diseases  Urumqi Xinjiang Uygur Autonomous Region China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"He","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology People's Hospital of Xinjiang Uygur Autonomous Region  Urumqi Xinjiang Uygur Autonomous Region China"},{"name":"Xinjiang Clinical Research Center for Digestive Diseases  Urumqi Xinjiang Uygur Autonomous Region China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology People's Hospital of Xinjiang Uygur Autonomous Region  Urumqi Xinjiang Uygur Autonomous Region China"},{"name":"Xinjiang Clinical Research Center for Digestive Diseases  Urumqi Xinjiang Uygur Autonomous Region China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8903-9440","authenticated-orcid":false,"given":"Feng","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology People's Hospital of Xinjiang Uygur Autonomous Region  Urumqi Xinjiang Uygur Autonomous Region China"},{"name":"Xinjiang Clinical Research Center for Digestive Diseases  Urumqi Xinjiang Uygur Autonomous Region China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)31288-5"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jso.26881"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00011-10"},{"key":"e_1_2_10_5_1","volume-title":"Gastric Cancer, StatPearls Publishing Copyright \u00a9 2022","author":"Recio\u2010Boiles A.","year":"2022"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575\u2010022\u201000591\u20100"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23031883"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiw031"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v19.i45.8192"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109096"},{"key":"e_1_2_10_11_1","first-page":"515","article-title":"Expression of Matrix Metalloproteinases MMP\u20102 and MMP\u20109 in Gastric Cancer and Their Relation to Claudin\u20104 Expression","volume":"23","author":"Lee L. 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