{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:57:46Z","timestamp":1791896266734,"version":"4.3.4"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,6,8]],"date-time":"2025-06-08T00:00:00Z","timestamp":1749340800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,6,8]],"date-time":"2025-06-08T00:00:00Z","timestamp":1749340800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In this study, novel thiosemicarbazone derivative molecules (\n                    <jats:bold>5a\u2013j<\/jats:bold>\n                    ) were synthesized. The characterization of the synthesized molecules was carried out using\n                    <jats:sup>1<\/jats:sup>\n                    H\u2013,\n                    <jats:sup>13<\/jats:sup>\n                    C\u2013NMR, FT\u2013IR, and Q\u2013TOF spectroscopic techniques. Then, the inhibitory effect of these compounds was examined for human carbonic anhydrase I and II (hCA I, and II) and acetylcholinesterase (AChE). The hCA I II, and AChE were efficiently inhibited by synthesized thiosemicarbazone derivatives, with Ki values in the range of 0.293\u20131.666\u00a0\u00b5M for hCA I, 0.298\u20131.332\u00a0\u00b5M for hCA II, and 52.51\u2013138.63\u00a0\u00b5M for AChE, respectively. Finally, the sensor properties of the synthesized thiosemicarbazone derivatives were investigated. The newly synthesized thiosemicarbazone derivative molecules exhibited high selectivity towards Pb\n                    <jats:sup>2+<\/jats:sup>\n                    ions. The sensor prepared with\n                    <jats:bold>5a<\/jats:bold>\n                    from these molecules had a Nernstian behavior of 28.8\u00a0\u00b1\u00a01.5\u00a0mV\/decade and a low detection limit of 2.86\u00a0\u00d7\u00a010\n                    <jats:sup>\u22126<\/jats:sup>\n                    \u00a0M in a wide concentration range of 1.0\u00a0\u00d7\u00a010\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u20131.0\u00a0\u00d7\u00a010\n                    <jats:sup>\u22125<\/jats:sup>\n                    \u00a0M. The newly proposed sensor also had a wide pH working range (5.0\u201310.0), a very fast response time (5\u00a0s), and good reproducibility. The newly fabricated sensors were used as indicator electrodes for the potentiometric titration of Pb\n                    <jats:sup>2+<\/jats:sup>\n                    ions with ethylenediaminetetraacetic acid (EDTA) and were successfully applied for the determination of Pb\n                    <jats:sup>2+<\/jats:sup>\n                    ion content in different water samples.\n                  <\/jats:p>","DOI":"10.1002\/bab.70001","type":"journal-article","created":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T00:34:23Z","timestamp":1749429263000},"page":"14-27","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Synthesis, Characterization, Biological Properties, and Sensor Applications of Novel Thiosemicarbazone 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