{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T01:48:20Z","timestamp":1780537700130,"version":"3.54.1"},"reference-count":43,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T00:00:00Z","timestamp":1751846400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T00:00:00Z","timestamp":1751846400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The absence of prompt and reliable diagnosis limits the eradication of Crimean\u2013Congo's hemorrhagic fever (CCHF) caused by\n                    <jats:italic>Hyalomma<\/jats:italic>\n                    . Although there have been several recent developments in different points of care and diagnosis, there has been a major drawback caused by the commercial unavailability and limited accessibility of economical serosurveillance assays that pose significant challenges. The primary objective of this study is to predict and design a novel synthetic biomarker composed of immunodominant B\u2010 and T\u2010cell lymphocytes (HTL) interleukin 10 (IL\u201010) epitopes using the antigenic, non\u2010toxigenic, and nonallergenic glycoprotein, nucleocapsid protein, and RNA\u2010dependent RNA polymerase (RdRp). Top highly ranked B\u2010cell epitopes as well as IL\u201010 inducing epitopes were predicted, screened, selected, and linked with linkers. Then, utilizing a variety of web servers, the biomarker predictions of antigenicity (0.5594), allergenicity (nonallergic), solubility (protein\u2013sol score: 0.623), and physiochemical properties (molecular weight of 56,004.26\u00a0Da, instability index of 28.33), secondary (SOPMA) and tertiary structures (AlphaFold2), 3D model refinement (GDT_HA score of 1.0000), and validations were done. Additionally, codon adaptation (G\u2013C of 48.93%, CAI 1.0) and in silico cloning in the pcDNA3 vector were done. The molecular docking (HDock) of the final biomarker with the selected genes was done to see the binding affinity. The model had a docking score of \u2212291.82, a confidence score of 0.9446, and a ligand RSMD of 66.24. For this multi\u2010epitope design to be employed in commercial serodiagnostic kits, further experimental evaluation is required for its development.\n                  <\/jats:p>","DOI":"10.1002\/bab.70026","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T04:25:54Z","timestamp":1751862354000},"page":"367-379","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Computational Modeling and Validation of Predicted Diagnostic Peptides for Crimean\u2013Congo's Disease"],"prefix":"10.1002","volume":"73","author":[{"given":"Elijah Kolawole","family":"Oladipo","sequence":"first","affiliation":[{"name":"Department of Microbiology, Laboratory of Molecular Biology, Immunology and Bioinformatics Adeleke University Ede Osun Nigeria"},{"name":"Division of Diagnostic Assay Development and Medical Artificial Intelligence Helix Biogen Institute Ogbomoso Oyo Nigeria"},{"name":"Division of Vaccine and Pharmacotherapies Design and Development Helix Biogen Institute Ogbomoso Oyo Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3049-5693","authenticated-orcid":false,"given":"Sola Peter","family":"Ogunmodede","sequence":"additional","affiliation":[{"name":"Division of Diagnostic Assay Development and Medical Artificial Intelligence Helix Biogen Institute Ogbomoso Oyo Nigeria"},{"name":"Department of Microbiology University of Lagos Lagos Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gladys Ayodele","family":"Adigun","sequence":"additional","affiliation":[{"name":"Division of Diagnostic Assay Development and Medical Artificial Intelligence Helix Biogen Institute Ogbomoso Oyo Nigeria"},{"name":"Osun State University Osogbo Osun Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mercy Temiloluwa","family":"Ogunlowo","sequence":"additional","affiliation":[{"name":"Division of Diagnostic Assay Development and Medical Artificial Intelligence Helix Biogen Institute Ogbomoso Oyo Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oluwatomi Jeremiah","family":"Dada","sequence":"additional","affiliation":[{"name":"Division of Diagnostic Assay Development and Medical Artificial Intelligence Helix Biogen Institute Ogbomoso Oyo Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enyo Othniel","family":"Friday","sequence":"additional","affiliation":[{"name":"Division of Diagnostic Assay Development and Medical Artificial Intelligence Helix Biogen Institute Ogbomoso Oyo Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isaiah Isiolaotan","family":"Iyanuoluwa","sequence":"additional","affiliation":[{"name":"Division of Diagnostic Assay Development and Medical Artificial Intelligence Helix Biogen Institute Ogbomoso Oyo Nigeria"},{"name":"Obafemi Awolowo University Ife Osun Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerry Ayobami","family":"Oluwasegun","sequence":"additional","affiliation":[{"name":"Division of Diagnostic Assay Development and Medical Artificial Intelligence Helix Biogen Institute Ogbomoso Oyo Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olawumi Elizabeth","family":"Akindiya","sequence":"additional","affiliation":[{"name":"Division of Diagnostic Assay Development and Medical Artificial Intelligence Helix Biogen Institute Ogbomoso Oyo 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