{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T17:23:18Z","timestamp":1775668998120,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T00:00:00Z","timestamp":1753056000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T00:00:00Z","timestamp":1753056000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Diabetes mellitus is a metabolic disorder characterized by increased oxidative stress, hyperglycemia, and associated implications that require suitable therapy approaches. One of the most effective approaches is to add fortified foods with multifunctional properties.\n                    <jats:italic>Withania somnifera<\/jats:italic>\n                    (Ashwagandha) root powder can help diabetics maintain stable blood glucose levels and reduce oxidative stress and inflammation. This study investigated the effects of fortified meal supplementation on STZ\u2010induced diabetic rats. 2\u2010month\u2010old male albino Wistar rats were divided into four groups as follows: Group 1 (Controls), Group II (Diabetic), Group III (Fortified Food), and Group IV (Diabetic + FF). Diabetes was induced by IP injection of Streptozotocin (STZ) at 50\u00a0mg\/kg b.wt. The study found that supplying STZ\u2010induced diabetics with fortified foods significantly improved fasting glucose, body weight, C\u2010peptide levels, HbA1c, and insulin levels, as well as altered lipid profiles. Furthermore, diabetic rats fed with a fortified diet exhibited significant improvement in urea, uric acid, and creatinine levels. In addition, diabetic rats had aberrant plasma sodium, potassium, and calcium levels. Furthermore, liver function tests revealed elevated levels of AST, ALT, ALP, and LDH enzymes; however, diabetic rats fed with a supplemented diet had these enzyme levels reduced to normal. Moreover, we observed increased lipid peroxidation and nitric oxide levels with altered antioxidant status (reduced glutathione, catalase, glutathione peroxidase, superoxide dismutase, and glutathione\n                    <jats:italic>S<\/jats:italic>\n                    \u2010transferase) in diabetic rats. Supplementation with fortified food decreased oxidative stress. Furthermore, fortified food supplementation to diabetic rats normalized the mRNA expression of PEPCK, G6Pase, IGFBP, GLUT\u20102, SREBP1c, ABCA1, ABCG1, and fatty acid synthase compared to diabetic rats. Our histopathology examinations confirmed these findings. Our findings revealed that fortified foods can be beneficial in diabetes management, reducing complications and increasing overall health outcomes. Fortified food, which is high in protein, minerals, and phytochemicals, provides an effective way to manage diabetes and promote overall health.\n                  <\/jats:p>","DOI":"10.1002\/bab.70031","type":"journal-article","created":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T09:09:52Z","timestamp":1753088992000},"page":"424-434","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fortified Food With\n                    <i>Withania Somnifera<\/i>\n                    Modulates Glucose Metabolism in STZ\u2010Induced Hyperglycemia in Rats"],"prefix":"10.1002","volume":"73","author":[{"given":"Chethan Kumar","family":"Narayanaswamy","sequence":"first","affiliation":[{"name":"Department of Biotechnology REVA University  Bengaluru Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gouthami","family":"Kuruvalli","sequence":"additional","affiliation":[{"name":"Department of Biotechnology REVA University  Bengaluru Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Subhasish","family":"Maity","sequence":"additional","affiliation":[{"name":"Department of Biotechnology REVA University  Bengaluru Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Althaf Hussain","family":"Shaik","sequence":"additional","affiliation":[{"name":"Department of Zoology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hymavathi","family":"Reddyvari","sequence":"additional","affiliation":[{"name":"Section of Thoracic Surgery, Lewis Katz School of Medicine Temple University  Philadelphia Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0077-6995","authenticated-orcid":false,"given":"Vaddi Damodara","family":"Reddy","sequence":"additional","affiliation":[{"name":"Department of Biotechnology REVA University  Bengaluru Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guruprasad","family":"NM","sequence":"additional","affiliation":[{"name":"Department of Biotechnology REVA University  Bengaluru Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.02.012"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21176275"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11154\u2010008\u20109101\u20105"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.43697"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010017\u20100135\u2010z"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.311401"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(14)60613\u20109"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu15245096"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics15041057"},{"issue":"8","key":"e_1_2_8_11_1","first-page":"1044","article-title":"Withania somnifera: A Review","volume":"64","author":"Singh N.","year":"2012","journal-title":"Journal of Pharmacy and Pharmacology"},{"issue":"10","key":"e_1_2_8_12_1","first-page":"1039","article-title":"Antioxidant and Anti\u2010inflammatory Activities of Withania somnifera","volume":"23","author":"Alam F.","year":"2020","journal-title":"Journal of Medicinal Food"},{"issue":"3","key":"e_1_2_8_13_1","first-page":"151","article-title":"Antidiabetic Activity of Withania somnifera Root Extract","volume":"8","author":"Raut A. A.","year":"2017","journal-title":"Journal of Ayurveda and Integrative Medicine"},{"issue":"2","key":"e_1_2_8_14_1","first-page":"242","article-title":"Effect of Withania somnifera on Glucose Tolerance in Normal and Alloxan\u2010Induced Diabetic Rats","volume":"125","author":"Andallu B.","year":"2009","journal-title":"Journal of Ethnopharmacology"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/1356893"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.17179\/excli2022\u20105720"},{"key":"e_1_2_8_17_1","unstructured":"\u201cFood Safety and Standards (Fortification of Foods) Regulations 2018 \u201d FSSAI https:\/\/fssai.gov.in\/upload\/uploadfiles\/files\/Gazette_Notification_Alcoholic_Beverages_05_04_2018.pdf."},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12291-010-0058-2"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2007.0694"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.4103\/0253\u20107613.81504"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.3164\/jcbn.16\u201016"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2016.10.021"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/0960327110396527"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10040595"},{"key":"e_1_2_8_25_1","first-page":"1","article-title":"The Lowry Method for Protein Quantitation","volume":"1","author":"Waterborg J. 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