{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T03:27:13Z","timestamp":1791862033153,"version":"4.3.4"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Lonicera japonica<\/jats:italic>\n                    Flos (LJF) possesses antiviral, antioxidant, and anti\u2010inflammatory properties, but its mechanisms against rheumatoid arthritis (RA) are not well understood. This study aims to clarify LJF's molecular mechanisms in treating RA. Active components and targets of LJF and RA were determined using databases such as TCMSP, GeneCards, and OMIM. Protein\u2013protein interaction (PPI) network analysis and pathway enrichment analysis (via KEGG as well as GO databases) were conducted to predict potential targets and pathways. Molecular docking identified potential targets of three specific LJF components. The anti\u2010arthritic effects of LJF were evaluated in vivo using a collagen\u2010induced arthritis (CIA) rat model. Seventeen active LJF components and 126 potential anti\u2010RA targets were identified. Key compounds include quercetin, luteolin, kaempferol, beta\u2010carotene, and beta\u2010sitosterol. Main targets are AKT1, TP53, JUN, TNF, IL6, CASP3, EGFR, RELA, IL1B, and VEGFA. KEGG analysis suggested the involvement of the PI3K\u2010AKT, TNF, and IL\u201017 pathways. Molecular docking demonstrated that \u03b2\u2010sitosterol, quercetin, and luteolin effectively bind to AKT1, IL6, JUN, TNF, and TP53. In vivo studies confirmed that LJF reduces pathological damage and inflammatory markers (TNF\u2010\u03b1 and IL\u20106) in a dose\u2010dependent manner, supporting its anti\u2010RA effects via AKT and RELA through PI3K\u2010AKT and NF\u2010\u03baB pathways. This research identified the PI3K\u2010AKT and NF\u2010\u03baB signaling pathways as key targets of LJF, highlighting their roles in its anti\u2010RA effects. These findings point to the possibility of advancing research and clinical use of LJF in RA treatment.\n                  <\/jats:p>","DOI":"10.1002\/bab.70051","type":"journal-article","created":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T06:12:10Z","timestamp":1758694330000},"page":"611-622","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Network Pharmacology and Experimental Evaluation of\n                    <i>Lonicera japonica<\/i>\n                    Flos in Rheumatoid Arthritis"],"prefix":"10.1002","volume":"73","author":[{"given":"Zihan","family":"Yu","sequence":"first","affiliation":[{"name":"College of Pharmacy Shandong University of Traditional Chinese Medicine Jinan China"},{"name":"Department of Rheumatology and Immunology Affiliated Hospital of Shandong University of Traditional Chinese Medicine Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonghui","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Endocrinology Affiliated Hospital of Shandong University of Traditional Chinese Medicine Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Traditional Chinese Medicine Department The People's Hospital of Zouping City Zouping China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangjin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Shandong Lvjin Biotechnology Co. 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