{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:41:54Z","timestamp":1787704914512,"version":"build-2784847793"},"reference-count":60,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:00:00Z","timestamp":1764374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:00:00Z","timestamp":1764374400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Age\u2010related mitochondrial dysfunction is a primary cause of muscle degeneration. We aimed to identify mitochondria\u2010related differentially expressed genes (MR\u2010DEGs) and construct a diagnostic model to improve the diagnosis of sarcopenia. Transcriptomic datasets GSE226151 (training) and GSE1428 (validation) were downloaded from GEO. Mitochondria\u2010related genes (MRGs) were sourced from human MitoCarta 3.0, and MR\u2010DEGs were screened as intersections of sarcopenia\u2010related genes, DEGs, and MRGs. Optimal biomarkers were selected using univariate logistic regression and LASSO regression. A diagnostic model was further estimated, and the diagnostic value was determined using receiver operating characteristic (ROC) curves. Finally, interaction networks and immune correlation analyses of the optimal MR\u2010DEGs were assessed. Six optimal MR\u2010DEGs (\n                    <jats:italic>ACOT11, BCO2, MRPL4, NDUFB9, UQCR10<\/jats:italic>\n                    , and\n                    <jats:italic>CASP8<\/jats:italic>\n                    ) were identified. The model achieved area under the curve (AUC) values of 0.935 and 0.899, respectively, in the GSE226151 and GSE1428. Significant immune correlations emerged; CD56\n                    <jats:sup>bright<\/jats:sup>\n                    natural killer cells and neutrophils were correlated with MR\u2010DEGs expression (all\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05). Network analysis revealed 20 co\u2010regulated genes that were significantly enriched in functions such as ATP synthesis\u2010coupled electron transport, mitochondrial respiration, and oxidative phosphorylation. Our six\u2010MR\u2010DEG diagnostic model demonstrated a robust clinical potential for sarcopenia diagnosis, with validation across platforms and significant pathophysiological relevance to mitochondrial immune dysregulation.\n                  <\/jats:p>","DOI":"10.1002\/bab.70084","type":"journal-article","created":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T05:45:26Z","timestamp":1764481526000},"page":"1125-1136","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Six\u2010Gene Signature Related to Mitochondrial Dysfunction as a Potential Diagnostic Biomarker for Sarcopenia"],"prefix":"10.1002","volume":"73","author":[{"given":"Xiaohuan","family":"Yang","sequence":"first","affiliation":[{"name":"Shanghai Yangpu District Shidong Hospital  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3144-1270","authenticated-orcid":false,"given":"Ming","family":"Tian","sequence":"additional","affiliation":[{"name":"Shanghai Burn Institute, Department of Burn, Ruijin Hospital Shanghai Jiaotong University School of Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyi","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of General Surgery Shanghai Sixth People's Hospital  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenke","family":"Lyu","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine The Second Hospital of Shanxi Medical University  Taiyuan Shanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12603-018-1139-9"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ageing\/afy106"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2022-067425"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glv070"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25042052"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP271212"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2023.112204"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21155236"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13578-024-01259-9"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1515\/hsz-2017-0331"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1249\/JES.0000000000000101"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1084531"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.601952"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.01.006"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2011-5225"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.26370"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00249.2004"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.1.207"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00059-023-05204-3"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-9-559"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv007"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1011"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/bimj.200900028"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.211"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn923"},{"key":"e_1_2_8_27_1","first-page":"2311","article-title":"Screening 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