{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T15:58:41Z","timestamp":1781798321658,"version":"3.54.5"},"reference-count":59,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:00:00Z","timestamp":1764374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:00:00Z","timestamp":1764374400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Bacterial leaf spot caused by\n                    <jats:italic>Xanthomonas citri<\/jats:italic>\n                    pv.\n                    <jats:italic>viticola<\/jats:italic>\n                    (\n                    <jats:italic>Xcv<\/jats:italic>\n                    ) in India is of economic importance. The objective of the investigation was to isolate and characterize bacterial antagonists from different tissues of the grapevine. Fifty endophytes were isolated from the leaves, stems, and roots of 15 different varieties of grapevine. The primary identification of the isolates was done by microscopic and biochemical characterizations. These endophytes were promoting the growth of the plants through both direct and indirect mechanisms. They were capable of producing various plant growth\u2010promoting traits, such as indole acetic acid and hydrolytic enzymes like cellulase, amylase, protease, and lipase. They were assessed for additional traits like phosphate solubilization, hydrogen cyanide production, and ammonia production. Further, these endophytes were screened for antagonistic activity against six\n                    <jats:italic>X. citri<\/jats:italic>\n                    pv.\n                    <jats:italic>viticola<\/jats:italic>\n                    isolated from different locations. Eleven out of 50 endophytic bacteria significantly inhibited\n                    <jats:italic>Xcv<\/jats:italic>\n                    , as evident from well\u2010diffusion methods. Three isolates, that is, EB7, EB26, and EB45, manifested the highest inhibition and were further screened for antibacterial activity using the detached leaf assay. The results showed that all three isolates have the highest potential to inhibit the\n                    <jats:italic>Xcv<\/jats:italic>\n                    , causing bacterial leaf spot. The molecular identification on the basis of 16S rRNA sequencing of the isolates EB7, EB26, and EB45 revealed that they were\n                    <jats:italic>Providencia vermicola<\/jats:italic>\n                    ,\n                    <jats:italic>Bacillus australimaris<\/jats:italic>\n                    , and\n                    <jats:italic>Bacillus zhangzhouensis<\/jats:italic>\n                    , respectively.\n                    <jats:italic>B. australimaris<\/jats:italic>\n                    and\n                    <jats:italic>B. zhangzhouensis<\/jats:italic>\n                    could be effectively used for the biological control of\n                    <jats:italic>Xcv<\/jats:italic>\n                    as they exhibited effective control in field trials.\n                  <\/jats:p>","DOI":"10.1002\/bab.70102","type":"journal-article","created":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T06:05:34Z","timestamp":1764482734000},"page":"1378-1389","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bio\u2010Intensive Role of Endophytic Bacteria for the Management of Bacterial Leaf Spot of Grapevine"],"prefix":"10.1002","volume":"73","author":[{"given":"Sneha","family":"Bhosale","sequence":"first","affiliation":[{"name":"MIT School of Bioengineering Sciences &amp; Research MIT ADT University  Loni Kalbhor Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sujoy","family":"Saha","sequence":"additional","affiliation":[{"name":"Plant Pathology, ICAR \u2010 National Research Centre for Grapes  Pune Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7468-3885","authenticated-orcid":false,"given":"Nayana","family":"Patil","sequence":"additional","affiliation":[{"name":"MIT School of Bioengineering Sciences &amp; Research MIT ADT University  Loni Kalbhor Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijayshree","family":"Chavan","sequence":"additional","affiliation":[{"name":"Plant Pathology, ICAR \u2010 National Research Centre for Grapes  Pune Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cropro.2009.12.010"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10658-020-01943-9"},{"key":"e_1_2_9_4_1","unstructured":"APEDA \u201cGrapes \u201dAPEDA India 2024 https:\/\/apeda.gov.in\/Grapes."},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1094\/PHYTO-06-12-0134-R"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.2136\/sssaj1995.03615995005900020031x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph191811256"},{"key":"e_1_2_9_8_1","first-page":"194","article-title":"The Efficacy of Windbreaks in Reducing the Spread of Citrus Canker Caused by Xanthamonas campestris pv. citri","volume":"72","author":"Gottwald T. 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