{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T15:58:41Z","timestamp":1781798321860,"version":"3.54.5"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T00:00:00Z","timestamp":1768694400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T00:00:00Z","timestamp":1768694400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Multidrug\u2010resistant tumor cells pose significant challenges in cancer treatment. Alternative strategies such as targeted gene silencing and the use of compounds with minimal cytotoxicity toward normal cells are therefore of great interest. Antimicrobial peptides (AMPs) have demonstrated anticancer potential due to their physicochemical properties. In lung cancer, overexpression of AKT serine\/threonine kinase 1 (AKT1) promotes abnormal tumor growth and progression. In this study, we synthesized chitosan\u2010based nanoparticles (CSNPs) co\u2010loaded with\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    peptide from strain P3 (\n                    <jats:italic>PAP3<\/jats:italic>\n                    ) (an AMP) and siRNA targeting the\n                    <jats:italic>AKT1 gene<\/jats:italic>\n                    , and evaluated their anticancer activity at the cellular and molecular levels. Characterization of the CSNPs revealed a nanoscale structure, low polydispersity index, and moderate encapsulation efficiency for both peptide and siRNA. Evaluation using L929 cells confirmed PAP3's nontoxic profile, while a dose\u2010dependent anticancer effect against A549 cells was observed. Delivery of encapsulated peptide, siRNA, and their combination increased cell death and induced morphological changes in A549 cells. Gene expression analysis showed upregulation of pro\u2010apoptotic markers (\n                    <jats:italic>Bax<\/jats:italic>\n                    and\n                    <jats:italic>Caspase\u20103)<\/jats:italic>\n                    and downregulation of the anti\u2010apoptotic marker\n                    <jats:italic>Bcl2<\/jats:italic>\n                    , indicating promising anticancer properties of the engineered compound. In conclusion, co\u2010delivery of PAP3 and AKT1\u2010targeting siRNA via CSNPs demonstrates potential for future anticancer therapies.\n                  <\/jats:p>","DOI":"10.1002\/bab.70123","type":"journal-article","created":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T07:27:55Z","timestamp":1768807675000},"page":"1602-1610","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<i>Pseudomonas aeruginosa<\/i>\n                    Peptide From Strain P3 (\n                    <i>PAP3<\/i>\n                    ) and AKT Serine\/Threonine Kinase 1 (\n                    <i>AKT1<\/i>\n                    ) siRNA\u2010Loaded Chitosan Nanoparticle as a Co\u2010Delivery System for Enhanced Anticancer Activity in Lung Cancer Cells"],"prefix":"10.1002","volume":"73","author":[{"given":"Shabeer","family":"Padariyakam","sequence":"first","affiliation":[{"name":"Amity Institute of Biotechnology Amity University Rajasthan  Jaipur Rajasthan India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nimisha R.","family":"Nair","sequence":"additional","affiliation":[{"name":"Athmic Biotech Solutions Pvt Ltd R&amp;D Lab  Trivandrum Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanker Lal","family":"Kothari","sequence":"additional","affiliation":[{"name":"Amity Institute of Biotechnology Amity University Rajasthan  Jaipur Rajasthan India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,18]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11051-020-04838-2"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2021.12762"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2017.1307212"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/psc.3550"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nanoso.2018.01.011"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929866524666170621093647"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25122850"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13568-020-01139-8"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2021.105784"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.12.032"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2017.10.010"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens11091044"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2016.02.032"},{"issue":"6","key":"e_1_2_11_15_1","first-page":"1305","article-title":"Suppression of Akt1 Expression by Small Interference RNA Inhibits SGC7901 Cell Growth in Vitro and in Vivo","volume":"22","author":"Cui X. 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