{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T13:58:54Z","timestamp":1789826334898,"version":"4.0.1"},"reference-count":54,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Multidrug\u2010resistant (MDR) microorganisms are widely recognized as a public health concern due to the antibiotic resistance, which is a major risk to global human health. Amino acids have been utilized to produce antimicrobial peptides as they are extremely effective and less likely to build microbial resistance. This study evaluates the efficacy of six aliphatic amino acids, isoleucine, methionine, valine, glycine, alanine, and proline against\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    . It is a Gram\u2010negative MDR pathogen that utilizes\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acyl\u2010homoserine lactones as signaling molecules to regulate the quorum sensing pathway and express virulence factors. The MIC and sub\u2010MIC were determined by the broth dilution method. This finding reveals that isoleucine exhibits a higher inhibitory effect on the organism at MIC and sub\u2010MIC concentration of amino acids compared to the inhibition by other amino acids. The biofilm production was inhibited at a higher percentage, and also EPS production was inhibited in treatment with isoleucine. Confocal laser scanning microscope results depicts the disintegration of biofilm formation upon treatment. The isoleucine\u2010treated culture of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    effectively inhibits the toxic pyocyanin production. Pyoverdines play a crucial role in iron acquisition, and their production was found to be inhibited by isoleucine. Motility was observed by swimming and swarming, and the results obtained from the gene expression studies demonstrated that isoleucine, methionine, valine, and glycine inhibited more virulence gene expression compared to alanine and proline.\n                  <\/jats:p>","DOI":"10.1002\/bab.70139","type":"journal-article","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T10:12:27Z","timestamp":1770027147000},"page":"1875-1884","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Influence of nonpolar aliphatic amino acids on\n                    <i>Pseudomonas aeruginosa<\/i>\n                    for virulence factors production"],"prefix":"10.1002","volume":"73","author":[{"given":"Arya","family":"Ramesh","sequence":"first","affiliation":[{"name":"Department of Biotechnology School of Bioengineering Faculty of Engineering and Technology SRM Institute of Science and Technology Kattankulathur 603203 Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abinaya","family":"Kanagaraja","sequence":"additional","affiliation":[{"name":"Department of Biotechnology School of Bioengineering Faculty of Engineering and Technology SRM Institute of Science and Technology Kattankulathur 603203 Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8347-4687","authenticated-orcid":false,"given":"Subash C. B.","family":"Gopinath","sequence":"additional","affiliation":[{"name":"Department of Neonatology Saveetha Institute of Medical and Technical Sciences (SIMATS) Saveetha Medical College &amp; Hospital Thandalam Tamil Nadu India"},{"name":"Faculty of Chemical Engineering &amp; Technology Universiti Malaysia Perlis (UniMAP) Arau Perlis Malaysia"},{"name":"Institute of Nano Electronic Engineering Universiti Malaysia Perlis (UniMAP) Kangar Perlis Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2739-4882","authenticated-orcid":false,"given":"Pachaiappan","family":"Raman","sequence":"additional","affiliation":[{"name":"Department of Biotechnology School of Bioengineering Faculty of Engineering and Technology SRM Institute of Science and Technology Kattankulathur 603203 Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,2]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1179\/2047773215Y.0000000030"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.21.287752"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/14787210.2021.1843427"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.99"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01944-18"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.001116"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2024.02.012"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2020.107671"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9060330"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.118407"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2022.127107"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02468-14"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fshw.2021.02.003"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2023.08.004"},{"key":"e_1_2_11_16_1","article-title":"Modulation of Quorum Sensing\u2010Controlled Virulence Factors in Chromobacterium violaceum by Selective Amino Acids","volume":"365","author":"Champalal L.","year":"2018","journal-title":"FEMS Microbiology Letters"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2024.104532"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.5740\/jaoacint.18-0251"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0038492"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep44195"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.1c07894"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10020165"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01059-09"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2012.08.002"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrep.2021.100972"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac60111a017"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-019-09618-0"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00592-21"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.14600"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-020-01909-4"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2022.02.024"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2018.12.189"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2022.2131626"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2008.09.015"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2023.106922"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/md20020085"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2011.06.001"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24244560"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-023-02169-0"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnx051"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology11121711"},{"issue":"2019","key":"e_1_2_11_42_1","article-title":"Peptide Mix From Olivancillaria hiatula Interferes With Cell\u2010to\u2010Cell Communication in Pseudomonas aeruginosa","author":"Gasu E. 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