{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:54:04Z","timestamp":1786118044370,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Nanocarriers have emerged as promising systems for improving drug\u2010delivery efficiency and advancing diagnostic strategies in cancer treatment.\n                    <jats:sc>l<\/jats:sc>\n                    \u2010asparaginase has well\u2010established antitumor and anti\u2010leukemic properties, but its therapeutic performance is often restricted due to its short biological half\u2010life and poor instability in the unmodified form. The present study investigated the encapsulation of\n                    <jats:sc>l<\/jats:sc>\n                    \u2010asparaginase on a green\u2010synthesized nanocarrier to enhance its overall therapeutic efficacy. The nanocarrier was produced utilizing citrus orange peel extract, which served simultaneously as a reducing, stabilizing, and capping material during the formation of zinc oxide\u2010doped selenium nanoparticles (ZnO\u2010Se\u2010NPs). This eco\u2010friendly synthesis method not only stabilized the nanoparticles but also incorporated naturally occurring phytochemicals, including flavonoids, phenolic compounds, ascorbic acid, and carotenoids\u2014that may contribute to additional pharmacological benefits. The synthesized\n                    <jats:sc>l<\/jats:sc>\n                    \u2010asparaginase encapsulated ZnO\u2010Se\u2010NPs (ZnO\u2010Se\u2010Asp) nanocomposite exhibited notable anticancer activity while maintaining low cytotoxicity. The detailed characterization of the nanoparticles was carried out utilizing UV\u2013Vis spectroscopy to confirm the presence of ZnO\u2010Se\u2010Asp, energy dispersive x\u2010ray (EDX), and scanning electron microscope (SEM) for morphological and elemental analysis. The Fourier transform infrared spectroscopy (FTIR) was studied to identify the functional groups, and x\u2010ray diffraction (XRD) was performed to study the crystallinity of synthesized ZnO\u2010Se\u2010Asp nanocomposite. The cytotoxic potential of synthesized ZnO\u2010Se\u2010Asp nanocomposite was estimated using the methyl thiazolyl tetrazolium (MTT) assay through the determined IC\n                    <jats:sub>50<\/jats:sub>\n                    value, as well as its effects on the cell viability and proliferation were assessed. Finally, intracellular reactive oxygen species (ROS) levels and Caspase\u20103 activity were performed to determine oxidative stress generation and apoptotic induction. Overall, the results indicated that the green\u2010synthesized ZnO\u2010Se\u2010Asp nanocomposite offers a promising platform capable of improving anticancer efficacy while offering better stability and bioavailability.\n                  <\/jats:p>","DOI":"10.1002\/bab.70147","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:32:52Z","timestamp":1772991172000},"page":"1965-1974","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biosynthesis and Characterization of Zinc Oxide\u2010Doped Selenium Nanocomposite Conjugated With\n                    <scp>l<\/scp>\n                    \u2010Asparaginase Against Human Hepatoma Cell Line"],"prefix":"10.1002","volume":"73","author":[{"given":"G.","family":"Baskar","sequence":"first","affiliation":[{"name":"Department of Biotechnology St. Joseph's College of Engineering  Chennai India"},{"name":"KU\u2010KIST Graduate School of Converging 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