{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:03:17Z","timestamp":1791378197635,"version":"4.3.2"},"reference-count":21,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:00:00Z","timestamp":1774224000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:00:00Z","timestamp":1774224000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Pneumococcal pneumonia, caused by\n                    <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n                    , has emerged as a global health threat, especially in children below 5 years of age and elderly population.\n                    <jats:italic>S. pneumoniae<\/jats:italic>\n                    remains a major global health threat which necessitates scalable and high\u2010yield vaccine production. This study presents an innovative fermentation platform for\n                    <jats:italic>S. pneumoniae<\/jats:italic>\n                    (serotype 18C) by optimizing an already refined media composition using data\u2010driven Design of Experiment (DoE) approach. Unlike conventional applications, Plackett\u2013Burman design (PBD) was strategically employed beyond screening, identifying statistically significant parameters and the interaction between key factors of dextrose, MgSO\n                    <jats:sub>4<\/jats:sub>\n                    , pH, and aeration. Results reveal that pH level of 7.1 significantly enhances the antigenic capsular polysaccharide yield. Further, ANOVA analysis confirmed a strong synergistic effect between dextrose and MgSO\n                    <jats:sub>4<\/jats:sub>\n                    . In addition, the present study also finds that fermentation at lower level of aeration (0.2 vvm) favors the bacterial growth aligning with\n                    <jats:italic>S. pneumoniae<\/jats:italic>\n                    \u2019s facultative anaerobic or microaerophilic nature. The downstream processing integrating centrifugation and diafiltration (100 kDa) improves the product recovery and purity reinforcing the process efficiency. The unconventional application of PBD combined with systematic optimization of refined process sets a new benchmark for pneumococcal fermentation strategies. The findings demonstrate that precise modulation of fermentation conditions can enhance the vaccine antigen yield addressing the scalability challenges in industrial production. Future work may focus on extending this DoE\u2010based platform to other serotypes of\n                    <jats:italic>S. pneumoniae<\/jats:italic>\n                    and refining fermentation kinetics for next generation pneumococcal vaccine. This study not only challenges the traditional experimental set up, but paves the way for adaptive, data\u2010driven bioprocess innovations in vaccine manufacturing.\n                  <\/jats:p>","DOI":"10.1002\/bab.70158","type":"journal-article","created":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T06:13:07Z","timestamp":1774332787000},"page":"2123-2134","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Optimization of Fermentation Conditions Using Design of Experiment (DoE) to Enhance the Production of Antigenic Capsular Polysaccharides From\n                    <i>Streptococcus pneumoniae<\/i>\n                    Serotype 18C for Pneumococcal Vaccine Development"],"prefix":"10.1002","volume":"73","author":[{"given":"Amol Dattatraya","family":"Mahajan","sequence":"first","affiliation":[{"name":"MIT School of Bioengineering Sciences &amp; Research MIT Art Design and Technology University  Pune India"},{"name":"Serum Institute of India Pvt. Ltd. (SIIPL)  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pramod Maruti","family":"Jadhav","sequence":"additional","affiliation":[{"name":"Serum Institute of India Pvt. Ltd. (SIIPL)  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avinash Rohidas","family":"Memane","sequence":"additional","affiliation":[{"name":"Serum Institute of India Pvt. Ltd. (SIIPL)  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gautam Kumar","family":"Jha","sequence":"additional","affiliation":[{"name":"Serum Institute of India Pvt. Ltd. (SIIPL)  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swapan Kumar","family":"Jana","sequence":"additional","affiliation":[{"name":"Serum Institute of India Pvt. Ltd. (SIIPL)  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prasad Deepak","family":"Dandekar","sequence":"additional","affiliation":[{"name":"MIT School of Bioengineering Sciences &amp; Research MIT Art Design and Technology University  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pranav Deepak","family":"Pathak","sequence":"additional","affiliation":[{"name":"MIT School of Bioengineering Sciences &amp; Research MIT Art Design and Technology University  Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,23]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006665"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00024\u201015"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010024\u201007626\u20103"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2018.1470726"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.21437"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciac475"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1871"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579\u2010018\u20100001\u20108"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/msystems.00025\u201020"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-323-02790-8.00003-2"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010micro\u2010091014\u2010104209"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41522\u2010024\u201000497\u20106"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00881\u201009"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.01.034"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI170501"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.4103\/jacp.jacp_29_16"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciae316"},{"key":"e_1_2_11_19_1","unstructured":"V.Kapare S. S.Kumar andA. A.Anand \u201cInternational Application Published Under the Patent Cooperation Treaty (PCT) \u201d (2013) WO 2013\/088448 A1."},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.seppur.2024.131301"},{"key":"e_1_2_11_21_1","unstructured":"Recommendations to Assure the Quality Safety and Efficacy of Pneumococcal Conjugate Vaccines Annex 3 TRS No. 977(World Health Organization 2013)."},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.5c00510"}],"container-title":["Biotechnology and Applied Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bab.70158","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bab.70158","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bab.70158","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T12:30:59Z","timestamp":1791376259000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/bab.70158"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,23]]},"references-count":21,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/bab.70158"],"URL":"https:\/\/doi.org\/10.1002\/bab.70158","archive":["Portico"],"relation":{},"ISSN":["0885-4513","1470-8744"],"issn-type":[{"value":"0885-4513","type":"print"},{"value":"1470-8744","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,23]]},"assertion":[{"value":"2025-04-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}