{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:03:55Z","timestamp":1791378235320,"version":"4.3.2"},"reference-count":60,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The enzymes and anticancer properties of disubstituted indole, indole\u2010imidazole, and indole\u2010pyrazole derivatives were determined. These building blocks are based on a privileged 3\u2010substituted indole scaffold that, in turn, has been used to explore potential effects for the enzymes and cancer cells. Inhibition studies for acetylcholinesterase (AChE), glutathione\n                    <jats:italic>S<\/jats:italic>\n                    \u2010transferase (GST), alpha\u2010amylase (\u03b1\u2010Amy), and alpha\u2010glucosidase (\u03b1\u2010Gly) were conducted. IC\n                    <jats:sub>50<\/jats:sub>\n                    values for the 3\u2010substituted indoles were identified as follows: AChE, 3.329\u00a0\u00b1\u00a00.975 to 5.544\u00a0\u00b1\u00a00.380\u00a0\u00b5M; GST, 2.228\u00a0\u00b1\u00a00.407 to 6.026\u00a0\u00b1\u00a01.100\u00a0\u00b5M; \u03b1\u2010Gly, 3.380\u00a0\u00b1\u00a00.616 to 5.330\u00a0\u00b1\u00a01.024\u00a0\u00b5M; and \u03b1\u2010Amy, 3.397\u00a0\u00b1\u00a00.472 to 5.173\u00a0\u00b1\u00a00.682\u00a0\u00b5M. Indole derivatives that exhibited strong enzyme inhibition were investigated for their cytotoxic effects on hepatocellular carcinoma (HepG2) and glioblastoma (U\u201087) cell lines at 24 and 48\u00a0h. Overall, it was found that some indole derivatives showed IC\n                    <jats:sub>50<\/jats:sub>\n                    values comparable to those of the standard drug, and throughout the process, some indole derivatives exhibited good IC\n                    <jats:sub>50<\/jats:sub>\n                    values, indicating good activity and low cytotoxic effects. The most active compounds were identified as compound 15 against GST (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a02.228\u00a0\u00b1\u00a00.407\u00a0\u00b5M), compounds 1 and 16 against AChE (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a03.329\u00a0\u00b1\u00a00.975 and 3.349\u00a0\u00b1\u00a00.845\u00a0\u00b5M), compounds 4 and 14 in HepG2 cells (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a033.13 and 36.71\u00a0\u00b5M at 48\u00a0h), and compounds 12 and 17 in U\u201087 cells (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u00a0=\u00a038.43 and 47.53\u00a0\u00b5M at 48\u00a0h). These indole\u2010based derivatives are also subjected to a structure\u2013activity relationship (SAR) study by the molecular docking method. Most indole derivatives showed enzyme inhibition results similar to, but below, those of the standard drug.\n                  <\/jats:p>","DOI":"10.1002\/bab.70166","type":"journal-article","created":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T07:15:15Z","timestamp":1775027715000},"page":"2210-2222","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multitarget Enzyme Inhibition and Anticancer Potential of 3\u2010Substituted Indole Derivatives Supported by Molecular Docking Studies"],"prefix":"10.1002","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4838-1503","authenticated-orcid":false,"given":"Adnan","family":"Cetin","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Education Van Yuzuncu Yil University  Van T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ercan","family":"Oguz","sequence":"additional","affiliation":[{"name":"Department of Medical Services and Techniques, Health Services Vocational School I\u011fd\u0131r University  I\u011fd\u0131r T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0538-3157","authenticated-orcid":false,"given":"Fikret","family":"T\u00fcrkan","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences, Faculty of Dentistry I\u011fd\u0131r University  I\u011fd\u0131r T\u00fcrkiye"},{"name":"Department of Basic Medical Science, Medical of Faculty Nakhchivan State University  Nakhchivan Azerbaijan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5620-1800","authenticated-orcid":false,"given":"Karolina","family":"Babijczuk","sequence":"additional","affiliation":[{"name":"Department of Bioactive Products, Faculty 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