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Thus, we synthesized zinc oxide nanoparticles from\n                    <jats:italic>Prunus nepalensis<\/jats:italic>\n                    and studied the effect of zinc oxide nanoparticles (PNZnONPs) on the inhibition of breast cancer cell lines (MCF\u20107 and MCF10A). UV\u2012Vis, SEM, and XRD analytical methods were used for nanoparticle characterization. MCF10A and MCF\u20107 cells were treated with six different doses of PNZnONPs (5, 10, 20, 40, 50, and 100\u00a0\u00b5g\/mL) for the cell viability study by MTT assay. Moreover, two doses (40 and 100\u00a0\u00b5g\/mL) were used for the qRT\u2010PCR, and 100\u00a0\u00b5g\/mL was used for western blotting studies to determine the levels of mRNA gene and protein expression, respectively. Furthermore, molecular docking studies revealed that protein\u2012ligand interactions were significantly effective in inhibiting the proliferation of breast cancer cells. All the studied bioactive compounds showed better responses in ADMET studies. Both the mRNA and protein levels of the tumor suppressor gene p53 and the apoptotic gene Bax were upregulated in PNZnONP\u2010treated MCF\u20107 cells. PNZnONPs stimulated caspase\u20103 enzyme activity and DNA fragmentation in MCF\u20107 cells and thus showed potential significant activity against cancer cell proliferation.\n                  <\/jats:p>","DOI":"10.1002\/bab.70170","type":"journal-article","created":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T04:14:46Z","timestamp":1775621686000},"page":"2259-2276","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Docking and Anticancerous Activity of Zinc Oxide Nanoparticles Synthesized From Fruits of\n                    <i>Prunus nepalensis<\/i>\n                    Extract"],"prefix":"10.1002","volume":"73","author":[{"given":"Samuel Shiferaw","family":"Biresaw","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Sharda School of Bioscience and Technology Sharda University  Greater Noida India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paratpar","family":"Sarkar","sequence":"additional","affiliation":[{"name":"Department of Chemistry &amp; Biochemistry, Sharda School of Engineering &amp; Science Sharda University  Greater Noida India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pankaj","family":"Taneja","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Sharda School of Bioscience and Technology Sharda University  Greater Noida India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neetu Kumra","family":"Taneja","sequence":"additional","affiliation":[{"name":"Department of Microbiology NIFTEM  Sonipat India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivek","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Department of Chemistry &amp; Biochemistry, Sharda School of Engineering &amp; Science Sharda University  Greater Noida India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dibyajit","family":"Lahiri","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Institute of Engineering and Management Kolkata University of Engineering and Management Kolkata  Kolkata India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moupriya","family":"Nag","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Institute of Engineering and Management Kolkata University of Engineering and Management Kolkata  Kolkata India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers3033279"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.9b00136"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470\u20102045(06)70793\u20108"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/nn800596w"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano5031317"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scp.2019.100179"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201060541\u20101"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0144280"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/87559129.2020.1854781"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11130\u2010015\u20100498\u20101"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00680"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/1750\u20103841.13398"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10787\u2010021\u201000799\u20107"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1515\/chem\u20102020\u20100121"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu12071966"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfca.2014.10.007"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22020499"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11130\u2010009\u20100135\u2010y"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2014.10.145"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfoodeng.2018.06.028"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfca.2015.10.002"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/01635581.2020.1817514"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0155264"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1517\/17425247.2010.502560"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045\u2010019\u20100833\u20103"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/1062562"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2019.110160"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molonc.2014.08.001"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2019.111624"},{"key":"e_1_2_10_31_1","first-page":"845","article-title":"Zinc Oxide Nanoparticles Selectively Induce Apoptosis in human Cancer Cells Through Reactive Oxygen Species","volume":"7","author":"Akhtar M. 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