{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T12:31:41Z","timestamp":1786365101181,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Marine environments are an abundant source of sustainable polymers with considerable biomedical potential. In this study, a marine\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    sp. strain KAR3 was isolated from the Gulf of Mannar. Extracellular polysaccharide (EPS) was isolated, characterized, and evaluated for its antibacterial, antibiofilm, and anticancer activity against MDA\u2010MB\u2010231 cells. Strain identification using biochemical characterization and 16S\n                    <jats:italic>rRNA<\/jats:italic>\n                    gene sequencing (Accession: PP330035) showed 100% similarity to\n                    <jats:italic>Streptomyces geysiriensis<\/jats:italic>\n                    , and phylogenetic analysis supported its affiliation with the\n                    <jats:italic>S. geysiriensis<\/jats:italic>\n                    clad.\n                    <jats:italic>S. geysiriensis<\/jats:italic>\n                    strain KAR3 produced 4.4\u00a0g\/L of EPS in the media after 7 days. EPS production was optimized in several culture media and structurally characterized by UV spectroscopy, Fourier transform infrared (FTIR),\n                    <jats:sup>1<\/jats:sup>\n                    H and\n                    <jats:sup>13<\/jats:sup>\n                    C nuclear magnetic resonance (NMR), and GC\u2013MS. FTIR analysis revealed C\u2013H and C = O stretching and glycosidic linkages.\n                    <jats:sup>1<\/jats:sup>\n                    H and\n                    <jats:sup>13<\/jats:sup>\n                    C NMR shifts aligned with sugar moieties, indicating the EPS is a complex polysaccharide. GC\u2013MS showed glucose as the major monosaccharide in the EPS. The isolated EPS exhibited dose\u2010dependent inhibition, with the largest zone of inhibition observed at 100\u00a0\u00b5g\/mL. The EPS exhibited notable antibacterial and biofilm inhibitory activity against\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    ,\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    ,\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    , and\n                    <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    in a concentration\u2010dependent manner. The minimum inhibitory concentration (MIC) calculated for the most susceptible strains was at 100\u00a0\u00b5g\/mL. The IC\n                    <jats:sub>50<\/jats:sub>\n                    value of EPS was found to be 25.44\u00a0\u00b5g\/mL, indicating a strong cytotoxic effect against MDA\u2010MB\u2010231 cancer cells. Together, these findings demonstrate the broad\u2010spectrum antibacterial and anticancer activity of marine EPS from\n                    <jats:italic>S. geysiriensis<\/jats:italic>\n                    and its potential as a natural source in the search for new antimicrobial compounds.\n                  <\/jats:p>","DOI":"10.1002\/bab.70192","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T11:58:44Z","timestamp":1786363124000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Extracellular Polysaccharide From Marine\n                    <i>Streptomyces<\/i>\n                    sp. KAR3: Structural Characterization, Antibacterial, and Anticancer Potential"],"prefix":"10.1002","author":[{"given":"Karthick Kumar","family":"S. B.","sequence":"first","affiliation":[{"name":"Department of Microbiology Karpagam Academy of Higher Education, Eachanari  Coimbatore Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7786-2034","authenticated-orcid":false,"given":"Rajkumar","family":"Prabhakaran","sequence":"additional","affiliation":[{"name":"School of Applied Bioscience, Food, and Agritech Rathinam Global Deemed to be University  Coimbatore Tamil Nadu India"},{"name":"Center for Cancer Research Karpagam Academy of Higher Education, Eachanari  Coimbatore Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4352-6630","authenticated-orcid":false,"given":"Rajkumar","family":"Manickam","sequence":"additional","affiliation":[{"name":"Center for Cancer Research Karpagam Academy of Higher Education, Eachanari  Coimbatore Tamil Nadu India"},{"name":"Department of Biotechnology, Centre for Cancer Research Karpagam Academy of Higher Education, Eachanari  Coimbatore Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karthikeyan","family":"Mahendran","sequence":"additional","affiliation":[{"name":"Department of Microbiology PSG College of Arts &amp; Science  Coimbatore Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4971-1642","authenticated-orcid":false,"given":"Ramkumar","family":"Muthu","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Sri Kaliswari College (Autonomous)  Sivakasi Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7116-6250","authenticated-orcid":false,"given":"Deenathayalan","family":"Uvarajan","sequence":"additional","affiliation":[{"name":"Center for Cancer Research Karpagam Academy of Higher Education, Eachanari  Coimbatore Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akilandeswari","family":"Periyasamy","sequence":"additional","affiliation":[{"name":"Department of Microbiology Karpagam Academy of Higher Education, Eachanari  Coimbatore Tamil Nadu 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