{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:26:33Z","timestamp":1787030793692,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Lignocellulosic residues are non\u2010cost, non\u2010edible, renewable feedstocks that can be used in polyhydroxybutyrate (PHB) production. PHB is a bioplastic synthetized by microorganisms, formed intracellularly under stressful growth conditions; however, its production costs are high, representing the major drawback for fossil\u2010based plastics replacement. The use of lignocellulosic waste in PHB production can help to reduce its costs. Therefore, this work aimed to optimize the second\u2010generation (2G) PHB production process from the lignocellulosic corn fraction (LCF). The LCF is a lignocellulosic by\u2010product generated during first\u2010generation (1G) PHB production from milled corn (\n                    <jats:italic>Zea mays<\/jats:italic>\n                    ). A novel integrated citric acid pretreatment followed by enzymatic hydrolysis in just one\u2010pot was employed. Two cellulolytic enzymes from a highly productive strain were employed at an enzyme loading of 11.64\u00a0mg \u03b2\u2010glucosidase\/g substrate and 0.6\u00a0mg cellulase\/g substrate. These enzymes concentrations were employed in a hydrolysis assay in an agitated tank, reaching 47.37\u00a0g\/L of total sugars (95.71% of glucose). This hydrolysate was employed as a low\u2010cost carbon source for 2G PHB production by\n                    <jats:italic>Cupriavidus necator<\/jats:italic>\n                    LPB2327. In the optimized condition, 8.90\u00a0g\/L of dry cell weight (DCW), and 7.64\u00a0g PHB\/L (85.89% PHB accumulation) were achieved. Overall, high product yields of 0.254\u00a0g PHB\/g substrate, and 0.127\u00a0g PHB\/g LCF were obtained. These results indicate the feasibility of using this integrated approach for 2G PHB production within a corn biorefinery strategy.\n                  <\/jats:p>","DOI":"10.1002\/bab.70197","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:25:16Z","timestamp":1787027116000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Second\u2010Generation Polyhydroxybutyrate Production From Pretreated Corn Lignocellulosic Fraction as a Strategy to Repurpose by Products From the Corn\u2010Processing Chain"],"prefix":"10.1002","author":[{"given":"Clara Matte Borges","family":"Machado","sequence":"first","affiliation":[{"name":"Department of Bioprocess Engineering and Biotechnology, Centro Polit\u00e9cnico Federal University of Paran\u00e1  Curitiba Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana Porto de Souza","family":"Vandenberghe","sequence":"additional","affiliation":[{"name":"Department of Bioprocess Engineering and Biotechnology, Centro Polit\u00e9cnico Federal University of Paran\u00e1  Curitiba Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariane F\u00e1tima Murawski de","family":"Mello","sequence":"additional","affiliation":[{"name":"Department of Bioprocess Engineering and Biotechnology, Centro Polit\u00e9cnico Federal University of Paran\u00e1  Curitiba Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo Amaro","family":"Bittencourt","sequence":"additional","affiliation":[{"name":"Department of Bioprocess Engineering and Biotechnology, Centro Polit\u00e9cnico Federal University of Paran\u00e1  Curitiba Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra M.","family":"Rozhkova","sequence":"additional","affiliation":[{"name":"Federal Research Centre Fundamentals of Biotechnology of the Russian Academy of Sciences  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan N.","family":"Zorov","sequence":"additional","affiliation":[{"name":"Federal Research Centre Fundamentals of Biotechnology of the Russian Academy of Sciences  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan Grace","family":"Karp","sequence":"additional","affiliation":[{"name":"Department of Bioprocess Engineering and Biotechnology, Centro Polit\u00e9cnico Federal University of Paran\u00e1  Curitiba Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vittoria Gomes","family":"Michelin","sequence":"additional","affiliation":[{"name":"Department of Bioprocess Engineering and Biotechnology, Centro Polit\u00e9cnico Federal University of Paran\u00e1  Curitiba Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Ricardo","family":"Soccol","sequence":"additional","affiliation":[{"name":"Department of Bioprocess Engineering and Biotechnology, Centro Polit\u00e9cnico Federal University of Paran\u00e1  Curitiba Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40643\u2010017\u20100137\u20109"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2020.122848"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2024.110403"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13065\u2010017\u20100251\u2010y"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2021.126074"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2022.115178"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/bbb.2195"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010020\u201010682\u20100"},{"key":"e_1_2_8_10_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2022.128537","article-title":"Polyhydroxybutyrate Production by Cupriavidus necator in a Corn Biorefinery Concept","volume":"370","author":"Mello A. F. M.","year":"2023","journal-title":"Bioresource Technology"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biteb.2021.100875"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/polym12122908"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jctb.2392"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2019.12.181"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2020.04.082"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibiod.2017.10.001"},{"key":"e_1_2_8_17_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.biortech.2023.130078","article-title":"Polyhydroxyalkanoates Production in Biorefineries: A Review on Current Status, Challenges and Opportunities","volume":"393","author":"Mello A. F. M.","year":"2024","journal-title":"Bioresource Technology"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2021.126165"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymdegradstab.2010.01.021"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2026.150193"},{"key":"e_1_2_8_21_1","unstructured":"C. M. B.Machado L. P.Vandenberghe S.de A. F. M.deMello C. R.Soccol andS. G.Karp \u201cProcesso integrado de pr\u00e9\u2010tratamento e sacarifica\u00e7\u00e3o enzim\u00e1tica em uma \u00fanica opera\u00e7\u00e3o unit\u00e1ria para obten\u00e7\u00e3o de a\u00e7\u00facares fermentesc\u00edveis a partir da fra\u00e7\u00e3o lignocelul\u00f3sica do milho (Integrated Process of Pretreatment and Enzymatic Saccharification in One\u2010Step for fermentable sugars obtention from the lignocellulosic corn fraction)\u201d. Patent Number BR 102024024561\u20100 A2. Deposited on 26th of November 2024. Published on 21st of October 2025."},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/agronomy10091348"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2021.124888"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac60147a030"},{"key":"e_1_2_8_25_1","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.biortech.2012.11.088","article-title":"Evaluation of by\u2010Products From the Biodiesel Industry as Fermentation Feedstock for Poly(3\u2010Hydroxybutyrate\u2010Co\u20103\u2010Hydroxyvalerate) Production by Cupriavidus necator","volume":"130","author":"Garc\u00eda I. 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