{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:26:58Z","timestamp":1787030818286,"version":"build-2736575974"},"reference-count":54,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Global warming and industrial pollutants pose significant threats to environmental sustainability. In response, enzymes have emerged as promising tools for mitigating pollution. Enzymatic hydrolysis is considered environmentally sustainable due to its biological nature. In Brazil, the estimated agro\u2010industrial waste production is an abundant source of lignocellulosic biomass, which contains cellulose, hemicellulose, and lignin. Its effective degradation requires a synergistic cocktail of enzymes, including cellulases, hemicellulases, and lignin\u2010degrading enzymes. Within this system, \u03b2\u2010glucosidases play a key role as a limiting factor in cellulose conversion, acting synergistically with other enzymes but being inhibited by glucose. This study aimed to produce and characterize \u03b2\u2010glucosidases from the crude extract of the cyanobacterium\n                    <jats:italic>Microcystis aeruginosa<\/jats:italic>\n                    CACIAM 03. Enzyme production occurred after 10 days of incubation. The crude enzymatic extract exhibited optimal activity at pH 4.5 and 40\u00b0C (28.51\u00a0U\/mL), high thermostability at 50\u00b0C (60% activity retained after 480\u00a0min), and tolerance to methanol (\u223c15% loss). The presence of Zn\n                    <jats:sup>2+<\/jats:sup>\n                    , Cl\n                    <jats:sup>2+<\/jats:sup>\n                    , and Mn\n                    <jats:sup>2+<\/jats:sup>\n                    increased enzymatic activity by 2\u20133\u00d7. Using 300\u00a0\u00b5g of total protein from the crude enzymatic extract, the enzyme demonstrated high affinity for cellobiose, with  of 0.156\u00a0\u00b1\u00a00.02\u00a0mM and  of 0.246\u00a0\u00b1\u00a00.01\u00a0\u00b5M\/min. These results reinforce the promising enzymatic potential of\n                    <jats:italic>M. aeruginosa<\/jats:italic>\n                    strains isolated from the Amazon region for sustainable biotechnological applications.\n                  <\/jats:p>","DOI":"10.1002\/bab.70200","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:39:32Z","timestamp":1787027972000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biochemical Studies and Production Optimization of \u03b2\u2010Glucosidase From\n                    <i>Microcystis aeruginosa<\/i>\n                    CACIAM 03 Using Organic Waste"],"prefix":"10.1002","author":[{"given":"Gustavo Marques","family":"Serra","sequence":"first","affiliation":[{"name":"Graduate Program in Biotechnology Institute of Biological Sciences Federal University of Par\u00e1  Bel\u00e9m Brazil"},{"name":"Laboratory of Biotechnology of Enzymes and Biotransformations Federal University of Par\u00e1  Bel\u00e9m Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matheus Silva da Fonseca","family":"Diniz","sequence":"additional","affiliation":[{"name":"Graduate Program in Biotechnology Institute of Biological Sciences Federal University of Par\u00e1  Bel\u00e9m Brazil"},{"name":"Laboratory of Biotechnology of Enzymes and Biotransformations Federal University of Par\u00e1  Bel\u00e9m Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evonnildo Costa","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Graduate Program in Biotechnology Institute of Biological Sciences Federal University of Par\u00e1  Bel\u00e9m Brazil"},{"name":"Laboratory of Biomolecular Technology Federal University of Par\u00e1  Bel\u00e9m Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agenor Valadares","family":"Santos","sequence":"additional","affiliation":[{"name":"Graduate Program in Biotechnology Institute of Biological Sciences Federal University of Par\u00e1  Bel\u00e9m Brazil"},{"name":"Laboratory of Biotechnology of Enzymes and Biotransformations Federal University of Par\u00e1  Bel\u00e9m Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana Pereira","family":"Xavier","sequence":"additional","affiliation":[{"name":"Graduate Program in Biotechnology Institute of Biological Sciences Federal University of Par\u00e1  Bel\u00e9m Brazil"},{"name":"Laboratory of Biotechnology of Enzymes and Biotransformations Federal University of Par\u00e1  Bel\u00e9m Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,17]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1088\/1742\u20106596\/1831\/1\/012033"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2021.125042"},{"key":"e_1_2_12_4_1","first-page":"1","volume-title":"Cellulose Chemistry and Properties: Fibers, Nanocelluloses and Advanced Materials","author":"Heinze 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