{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T05:18:45Z","timestamp":1787635125534,"version":"build-2736575974"},"reference-count":47,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Ionic liquids (ILs) are promising media for biotechnological applications, although their use in microbial systems is limited by their toxicity to living cells and biomacromolecules. In this study, the toxicity profiles of selected IL\u2010derived ions were evaluated toward\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    ,\n                    <jats:italic>Yarrowia lipolytica<\/jats:italic>\n                    , and\n                    <jats:italic>Kluyveromyces marxianus<\/jats:italic>\n                    by determining the maximum non\u2010toxic concentration (MNTC), and the experimental trends were further investigated through molecular docking against the corresponding yeast hexokinases. The results revealed a species\u2010dependent response, with\n                    <jats:italic>Y. lipolytica<\/jats:italic>\n                    displaying substantially higher tolerance than\n                    <jats:italic>K. marxianus<\/jats:italic>\n                    and\n                    <jats:italic>S. cerevisiae<\/jats:italic>\n                    . For the anionic series, toxicity increased consistently from Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    to [NTf\n                    <jats:sub>2<\/jats:sub>\n                    ]\n                    <jats:sup>\u2212<\/jats:sup>\n                    in the three yeasts, with [NTf\n                    <jats:sub>2<\/jats:sub>\n                    ]\n                    <jats:sup>\u2212<\/jats:sup>\n                    showing the lowest MNTC values in all cases. The cationic series also showed clear differences in toxicity, though in a more species\u2010dependent manner. Docking analyses revealed a strong qualitative relationship between binding affinity and MNTC, particularly for the anions, indicating that ions with more favourable interactions with hexokinase were generally tolerated only at lower concentrations. Thus, the results indicate that IL toxicity in yeasts emerges from the interplay between ion\u2010specific properties and species\u2010dependent structural features of the target enzyme. By integrating microbiological and computational analyses, this study provides new insight into the molecular basis of ion\u2010dependent and species\u2010dependent IL toxicity in yeasts of biotechnological relevance.\n                  <\/jats:p>","DOI":"10.1002\/bab.70204","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T04:23:28Z","timestamp":1787631808000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ionic Liquid Toxicity in Biotechnological Yeasts: Experimental Assessment and Molecular Docking Insights"],"prefix":"10.1002","author":[{"given":"Filipe S.","family":"Buarque","sequence":"first","affiliation":[{"name":"Biochemical Engineering Department School of Chemistry Federal University of Rio de Janeiro  Rio de Janeiro Brazil"},{"name":"CICECO\u2010Aveiro Institute of Materials Department of Chemistry University of Aveiro  Aveiro Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariane G.","family":"Santos","sequence":"additional","affiliation":[{"name":"Biochemical Engineering Department School of Chemistry Federal University of Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernardo D.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Biochemical Engineering Department School of Chemistry Federal University of Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matheus M.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering CERES University of Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mara G.","family":"Freire","sequence":"additional","affiliation":[{"name":"CICECO\u2010Aveiro Institute of Materials Department of Chemistry University of Aveiro  Aveiro Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Alice Z.","family":"Coelho","sequence":"additional","affiliation":[{"name":"Biochemical Engineering Department School of Chemistry Federal University of Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/pr11010031"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/D5GC06719G"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26164791"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.6b00562"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molliq.2024.124095"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2015.03.005"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s41061\u2010017\u20100110\u20102"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2022.108055"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jctb.4837"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcb.8b03733"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068\u2010015\u20100206\u20102"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep19889"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/pr7070471"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19030887"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2024.11.016"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/c4ra05295a"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2019.03.003"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934\u2010016\u20100417\u20107"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1134\/S0006297924030064"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/PR8091163"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.9b02128"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1039\/c1gc15140a"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.595074"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2012.01.030"},{"key":"e_1_2_9_26_1","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1016\/S1386-1425(98)00315-1","article-title":"Inhibition of Yeast Hexokinase: A Kinetic and Phosphorus Nuclear Magnetic Resonance Study","volume":"55","author":"Le Willson M.","year":"1999","journal-title":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.2c01956"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcb.1c00592"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22115612"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2012.06.020"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.polymdegradstab.2021.109751"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/je2010787"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molliq.2022.119796"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2019.00347"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42004\u2010020\u20100302\u20105"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.joc.2c02001"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.estlett.5c00688"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jct.2020.106340"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.3064"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201710782"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.procbio.2026.04.007"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.2816"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12551\u2010018\u20100407\u20106"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201085837\u20108"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22179282"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10295\u2010019\u201002228\u20109"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssuschemeng.5b00965"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1080\/01614940.2022.2074359"}],"container-title":["Biotechnology and Applied Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bab.70204","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bab.70204","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bab.70204","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T04:23:29Z","timestamp":1787631809000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1002\/bab.70204"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,24]]},"references-count":47,"alternative-id":["10.1002\/bab.70204"],"URL":"https:\/\/doi.org\/10.1002\/bab.70204","archive":["Portico"],"relation":{},"ISSN":["0885-4513","1470-8744"],"issn-type":[{"value":"0885-4513","type":"print"},{"value":"1470-8744","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,24]]},"assertion":[{"value":"2026-05-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"bab.70204"}}