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Lignocellulosic residues have received particular attention due to their abundance, renewability, and structural properties. Despite the growing interest in lignocellulosic residues as enzyme supports, sunflower stalks remain largely unexplored for lipase immobilization. This study aims to evaluate the use of sunflower stalks (\n                    <jats:italic>Helianthus annuus<\/jats:italic>\n                    L.), in natura and after alkaline and hydrothermal pretreatments, as supports for the immobilization of\n                    <jats:italic>Burkholderia cepacia<\/jats:italic>\n                    lipase by physical adsorption. Pretreatment improved enzyme performance, increasing thermal stability by up to 4.28\u2010fold compared to the free enzyme. The biocatalysts prepared using alkaline\u2010treated, hydrothermal\u2010treated, in natura supports, and free enzyme showed half\u2010life values of 19.95, 16.86, 10.25, and 4.66\u00a0h, respectively. Immobilization also enhanced enzyme reusability, particularly for the alkaline\u2010treated support, which maintained activity for up to six operational cycles. Kinetic analysis of the immobilized enzyme showed reduced\n                    <jats:italic>V<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    and apparent\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    values, suggesting changes in enzyme\u2013substrate interactions and possible diffusion limitations. These results indicate that pretreatment plays an important role in modifying the physicochemical properties of sunflower stalks, directly affecting the stability and reusability of the biocatalyst. Overall, this study highlights the potential of sunflower stalk residues as a low\u2010cost and effective support with improved catalytic performance and operational stability.\n                  <\/jats:p>","DOI":"10.1002\/bab.70209","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T06:01:28Z","timestamp":1790056888000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biobased Sunflower Stalk Matrix for Lipase Stabilization via Physical Adsorption"],"prefix":"10.1002","author":[{"given":"Jaci L.","family":"Vilanova Neta","sequence":"first","affiliation":[{"name":"Departamento de Ci\u00eancias Exatas Universidade Estadual de Santa Cruz  Ilh\u00e9us Bahia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa O.","family":"Jesus","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Engenharia Qu\u00edmica Universidade de Salvador\/Universidade Federal da Bahia  Salvador Bahia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sande A. S.","family":"Costa","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Engenharia de Processos Universidade Tiradentes  Aracaju Sergipe Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ed\u00e9lvio B.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Instituto Federal de Sergipe Campus Est\u00e2ncia  Est\u00e2ncia Sergipe Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita C. M.","family":"Miranda","sequence":"additional","affiliation":[{"name":"Departamento de Micologia, Centro de Bioci\u00eancias Universidade Federal de Pernambuco  Recife Pernambuco Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel P.","family":"Silva","sequence":"additional","affiliation":[{"name":"RENORBIO \u2013 Northeastern Biotechnology Network Federal University of Sergipe  S\u00e3o Crist\u00f3v\u00e3o Sergipe Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise S.","family":"Ruzene","sequence":"additional","affiliation":[{"name":"RENORBIO \u2013 Northeastern Biotechnology Network Federal University of Sergipe  S\u00e3o Crist\u00f3v\u00e3o Sergipe Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cleide M. 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