{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T14:03:04Z","timestamp":1790344984191,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Biodetergents represent sustainable alternatives to conventional chemical detergents, with lipases playing a key role in the removal of lipid\u2010based stains. This study aimed to optimize inducer conditions for enhanced lipase production by\n                    <jats:italic>Rhodotorula mucilaginosa<\/jats:italic>\n                    and to evaluate the enzyme's suitability for biodetergent applications. Lipase production was carried out in Yeast Nitrogen Base Broth supplemented with 1% (v\/v) different oil inducers under varying pH, temperature, and incubation time conditions. The highest lipase activity during production was achieved using olive oil and used cooking oil as inducers, reaching 3.80 and 3.75\u00a0U\/mL, respectively, at 72\u00a0h, pH 6, and 30\u00b0C. Biochemical characterization showed that the lipase exhibited optimal activity at pH 8 and 42\u00b0C, with a maximum activity of 3.25\u00a0U\/mL, and retained substantial stability under detergent\u2010relevant conditions. Enzyme activity was enhanced in the presence of Mg\n                    <jats:sup>2+<\/jats:sup>\n                    , reaching 4.67\u00a0U\/mL, whereas Zn\n                    <jats:sup>2+<\/jats:sup>\n                    and Ba\n                    <jats:sup>2+<\/jats:sup>\n                    strongly inhibited activity. In washing performance tests,\n                    <jats:italic>R. mucilaginosa<\/jats:italic>\n                    lipase achieved 29.40% oil removal, compared to 9.90% in the control and 54.53% for commercial\n                    <jats:italic>Thermomyces lanuginosus<\/jats:italic>\n                    lipase. These results demonstrate that inducer optimization significantly improves lipase production and highlight the potential of\n                    <jats:italic>R. mucilaginosa<\/jats:italic>\n                    lipase for biodetergent applications.\n                  <\/jats:p>","DOI":"10.1002\/bab.70211","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T13:31:59Z","timestamp":1790343119000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Inducer Optimization Enhances\n                    <i>Rhodotorula mucilaginosa<\/i>\n                    Lipase for Biodetergent Application"],"prefix":"10.1002","author":[{"given":"Ika Rahmatul","family":"Layly","sequence":"first","affiliation":[{"name":"Research Centre for Applied Microbiology National Research and Innovation Agency, BRIN  Cibinong, Bogor West Java Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catur","family":"Sriherwanto","sequence":"additional","affiliation":[{"name":"Research Centre for Applied Microbiology National Research and Innovation Agency, BRIN  Cibinong, Bogor West Java Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siswa","family":"Setyahadi","sequence":"additional","affiliation":[{"name":"Research Centre for Applied Microbiology National Research and Innovation Agency, BRIN  Cibinong, Bogor West Java Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edi","family":"Wahjono","sequence":"additional","affiliation":[{"name":"Research Centre for Applied Microbiology National Research and Innovation Agency, BRIN  Cibinong, Bogor West Java Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dewi","family":"Nandyawati","sequence":"additional","affiliation":[{"name":"Research Centre for Applied Microbiology National Research and Innovation Agency, BRIN  Cibinong, Bogor West Java Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kharis Yohan","family":"Abidin","sequence":"additional","affiliation":[{"name":"Research Centre for Applied Microbiology National Research and Innovation Agency, BRIN  Cibinong, Bogor West Java Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erma","family":"Widyasti","sequence":"additional","affiliation":[{"name":"Research Centre for Applied Microbiology National Research and Innovation Agency, BRIN  Cibinong, Bogor West Java Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Irfan","sequence":"additional","affiliation":[{"name":"Department of Biotechnology University of Sargodha  Sargodha Punjab Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12033\u2010022\u201000592\u2010z"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12668\u2010024\u201001319\u2010x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826068.2020.1719513"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1142536"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12010\u2010009\u20108547\u20106"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13213\u2010012\u20100546\u20100"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12010\u2010008\u20108293\u20101"},{"key":"e_1_2_9_9_1","unstructured":"YarrowandJ. 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