{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T05:02:58Z","timestamp":1790226178880,"version":"4.1.0"},"reference-count":58,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["financial code 001"],"award-info":[{"award-number":["financial code 001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Adsorption is the simplest method of enzyme immobilization, but it often requires additional treatments to improve stability. These treatments, however, may impact enzymatic activity. This study investigated the effect of crosslinking on the stability of \u03b2\u2010galactosidase immobilized by adsorption on A\u2010568 resin and its implications for enzymatic activity. The immobilized enzyme was crosslinked with different concentrations of glutaraldehyde (0.50% to 0.80% v\/v). Parameters such as thermal stability (30\u00b0C to 60\u00b0C), stability during consecutive reuse, and storage stability for 90\u00a0days at different pH values (3\u20138) were evaluated. The feasibility of using the immobilized enzyme in an industrial process was also evaluated, an aspect that is not usually addressed. The conversion capacity was evaluated in a continuous reactor at different feed flow rates (4\u201310\u00a0mL\/min). The results showed that crosslinking with glutaraldehyde (0.50%) provided greater stability for the enzyme adsorbed on the resin, which retained more than 90% of its initial activity at the end of the consecutive use cycle. Crosslinking improved the thermal stability of the enzyme, and its half\u2010life was more than doubled at all temperatures. Storage conditions affected the non\u2010crosslinked enzyme more, which showed intolerance to pH extremes, losing more than 60% of its initial activity at pH 3, 7, and 8, while the crosslinked enzyme retained more than 70% of its initial activity at all pH values after 90\u00a0days. Both enzymes showed great potential for industrial use in continuous processes. Although the cross\u2010Linked enzyme showed high operational stability, its lactose conversion was low.<\/jats:p>","DOI":"10.1002\/bab.70214","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T04:47:01Z","timestamp":1790225221000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Stabilization by Crosslinking of \u03b2\u2010Galactosidase Immobilized by Adsorption for Application in a Continuous Lactose Removal Process"],"prefix":"10.1002","author":[{"given":"Aliou Toro","family":"Lafia","sequence":"first","affiliation":[{"name":"School of Chemical Engineering Federal University of Uberl\u00e2ndia  Patos de Minas Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrielle Aparecia Paulista","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"School of Chemical Engineering Federal University of Uberl\u00e2ndia  Patos de Minas Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa Nayhara Soares Santana","family":"Falleiros","sequence":"additional","affiliation":[{"name":"School of Chemical Engineering Federal University of Uberl\u00e2ndia  Patos de Minas Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla Zanella","family":"Guidini","sequence":"additional","affiliation":[{"name":"School of Chemical Engineering Federal University of Uberl\u00e2ndia  Patos de Minas Minas Gerais 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