{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T07:49:47Z","timestamp":1791532187121,"version":"4.3.4"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32570034"],"award-info":[{"award-number":["32570034"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["iubmb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biotech and App Biochem"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Pichia pastoris<\/jats:italic>\n                    has emerged as a highly efficient heterologous protein expression system with significant potential for secretory production. However, its secretion efficiency is influenced by multiple factors, among which intracellular redox homeostasis plays a critical role. In this study, differential EGFP\u2010secreting strains (GS115\u2010High\/Low) were constructed via genomic integration of site\u2010directed mutations at\n                    <jats:italic>N<\/jats:italic>\n                    \u2010glycosylation sites in the \u03b1\u2010mating factor signal peptide of\n                    <jats:italic>P. pastoris<\/jats:italic>\n                    . Notably, the expression of the superoxide dismutase (\n                    <jats:italic>SOD1<\/jats:italic>\n                    ) gene was significantly upregulated in the high\u2010secretion strain GS115\u2010High. To further investigate the regulatory role of SOD1 in protein secretion, the\n                    <jats:italic>SOD1<\/jats:italic>\n                    gene was overexpressed in both high\u2010 and low\u2010secretion strains. Experimental results demonstrated that\n                    <jats:italic>SOD1<\/jats:italic>\n                    overexpression markedly enhanced EGFP secretion in the high\u2010secretion strain (\n                    <jats:italic>p\u00a0<\/jats:italic>\n                    \u2264\u00a00.05), but had no significant effect on the low\u2010secretion strain. Transcriptomic analysis revealed that amino acid metabolism pathways and oxidoreductase activity were significantly enhanced in the high\u2010secretion strain, suggesting a synergistic optimization of secretion efficiency through metabolic resource reallocation and antioxidant capacity. This study demonstrates that glycosylation modification and SOD1\u2010mediated redox regulation both contribute to enhanced EGFP secretion, providing a basis for a \u201cglycosylation\u2010antioxidant\u201d dual\u2010track optimization strategy for the rational design of industrial\n                    <jats:italic>P. pastoris<\/jats:italic>\n                    strains and high\u2010efficiency protein production.\n                  <\/jats:p>","DOI":"10.1002\/bab.70217","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T07:07:51Z","timestamp":1791529671000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enhancing the Secretion Efficiency of EGFP in\n                    <i>Pichia pastoris<\/i>\n                    Through\n                    <i>N<\/i>\n                    \u2010Glycosylation and SOD1\u2010Mediated Redox Regulation"],"prefix":"10.1002","author":[{"given":"Yaying","family":"Xie","sequence":"first","affiliation":[{"name":"Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, Engineering Research Center of Industrial Microbiology, College of Life Sciences Fujian Normal University  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxuan","family":"Lin","sequence":"additional","affiliation":[{"name":"Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, Engineering Research Center of Industrial Microbiology, College of Life Sciences Fujian Normal University  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujing","family":"Yu","sequence":"additional","affiliation":[{"name":"Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, Engineering Research Center of Industrial Microbiology, College of Life Sciences Fujian Normal University  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhui","family":"Wang","sequence":"additional","affiliation":[{"name":"Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, Engineering Research Center of Industrial Microbiology, College of Life Sciences Fujian Normal University  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Lin","sequence":"additional","affiliation":[{"name":"Affiliated People\u2019s Hospital, College of Integrative Medicine, Fujian\u2010Hong Kong\u2010Macau\u2010Taiwan Collaborative Laboratory for the Inheritance and Innovation of Traditional Chinese Medicine Fujian University of Traditional Chinese Medicine Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yide","family":"Huang","sequence":"additional","affiliation":[{"name":"Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, Engineering Research Center of Industrial Microbiology, College of Life Sciences Fujian Normal University  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,9]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"203","article-title":"Zygosaccharomyces Pastori, Nouvelle Esp\u00e8ce De Levures Copulation H\u00e9t\u00e9rogamique","volume":"36","author":"Guilliermond A.","year":"1920","journal-title":"Bulletin de la Soci\u00e9t\u00e9 Mycologique de France"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02538322"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.59.439"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574\u20106976.2000.tb00532.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.1059777"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751\u20107915.13322"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10930\u2010021\u201009968\u20107"},{"key":"e_1_2_9_9_1","doi-asserted-by":"crossref","DOI":"10.1093\/femsyr\/foab057","article-title":"Recombinant Protein Production in Pichia pastoris: From Transcriptionally Redesigned Strains to Bioprocess Optimization and Metabolic Modelling","volume":"21","author":"Erg\u00fcn B. 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