{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T06:00:31Z","timestamp":1784872831402,"version":"3.55.0"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T00:00:00Z","timestamp":1763337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T00:00:00Z","timestamp":1763337600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/V038303\/1"],"award-info":[{"award-number":["MR\/V038303\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br J Clin Pharmacol"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:p>\n                    MicroRNA\u2010122 (miR\u2010122) is a sensitive biomarker for paracetamol\u2010induced liver injury. This study assessed the diagnostic performance of dynamic chemical labelling (DCL) combined with an immunoassay platform to quantify miR\u2010122 in human serum, as a proof\u2010of\u2010concept for hospital use. This study used serum samples from 19 healthy individuals and 19 patients with paracetamol drug\u2010induced liver injury (DILI) collected in previous studies. miR\u2010122 was labelled with a biotinylated SMART\u2010Base and quantified using a multiplex, bead\u2010based, fluorescence detection system. The median miR\u2010122 concentration was significantly elevated at 233.4\u00a0pg\/mL (IQR: 74.5\u2013363.4) compared to 15.2\u00a0pg\/mL (IQR: 9.4\u201324) in the control group. The Mann\u2013Whitney\n                    <jats:italic>U<\/jats:italic>\n                    test showed significant group differences (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001). ROC analysis yielded an area under the curve (AUC) of 0.96, with a 29.7\u00a0pg\/mL cut\u2010off providing 92.6% sensitivity (95% CI: 76.6%\u201398.7%) and 89.4% specificity (95% CI: 68.6%\u201398.1%). miR\u2010122 moderately correlated with ALT (\n                    <jats:italic>r<\/jats:italic>\n                    \u2009=\u20090.56,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.03). DCL with fluorescence detection is a promising clinical tool for miR\u2010122 quantification.\n                  <\/jats:p>","DOI":"10.1002\/bcp.70364","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:19:46Z","timestamp":1763425186000},"page":"612-618","source":"Crossref","is-referenced-by-count":1,"title":["Short communication: Dynamic chemical labelling allows the measurement of the paracetamol toxicity biomarker microRNA\u2010122 in hospital clinical laboratories"],"prefix":"10.1002","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5119-3269","authenticated-orcid":false,"given":"Samar","family":"Alzeer","sequence":"first","affiliation":[{"name":"Centre for Cardiovascular Science, Queen's Medical Research Institute University of Edinburgh  Edinburgh UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0589-8927","authenticated-orcid":false,"given":"Antonio","family":"Marin\u2010Romero","sequence":"additional","affiliation":[{"name":"DESTINA Genomica S.L. Parque Tecnol\u00f3gico Ciencias de la Salud (PTS)  Granada Spain"},{"name":"Vircell S.L. Parque Tecnol\u00f3gico de la Salud  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9043-2445","authenticated-orcid":false,"given":"B\u00e1rbara","family":"L\u00f3pez\u2010Longarela","sequence":"additional","affiliation":[{"name":"DESTINA Genomica S.L. Parque Tecnol\u00f3gico Ciencias de la Salud (PTS)  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5475-931X","authenticated-orcid":false,"given":"Juan J.","family":"Guardia\u2010Monteagudo","sequence":"additional","affiliation":[{"name":"DESTINA Genomica S.L. Parque Tecnol\u00f3gico Ciencias de la Salud (PTS)  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6968-5672","authenticated-orcid":false,"given":"F. Javier","family":"Lopez\u2010Delgado","sequence":"additional","affiliation":[{"name":"DESTINA Genomica S.L. Parque Tecnol\u00f3gico Ciencias de la Salud (PTS)  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0259-9008","authenticated-orcid":false,"given":"Salvatore","family":"Pernagallo","sequence":"additional","affiliation":[{"name":"DESTINA Genomica S.L. Parque Tecnol\u00f3gico Ciencias de la Salud (PTS)  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3599-1954","authenticated-orcid":false,"given":"Juan J.","family":"Diaz\u2010Mochon","sequence":"additional","affiliation":[{"name":"GENYO Centre for Genomics and Oncological Research, Pfizer\/University of Granada\/Andalusian Regional Government  Granada Spain"},{"name":"Department of Medicinal and Organic Chemistry, Faculty of Pharmacy University of Granada  Granada Spain"},{"name":"Unit of Excellence in Chemistry Applied to Biomedicine and the Environment of the University of Granada  Granada Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria ibs. 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