{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T05:02:12Z","timestamp":1789794132827,"version":"4.0.1"},"reference-count":11,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100010767","name":"Innovative Medicines Initiative","doi-asserted-by":"publisher","award":["821283"],"award-info":[{"award-number":["821283"]}],"id":[{"id":"10.13039\/501100010767","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Br J Clin Pharmacol"],"abstract":"<jats:p>\n                    Drug\u2010induced liver injury (DILI) remains a major challenge in clinical practice and drug development, and reliable biomarkers are still needed. MicroRNA\u2010122 (miR\u2010122) has shown promise, but reported inter\u2010individual variability in healthy populations has raised concerns about its clinical utility. We analysed circulating miR\u2010122 levels in healthy volunteers across three European sites (Antwerp,\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u200996; Brussels,\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u2009124; and Leeds,\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u200960) using small RNA sequencing within the TransBioLine programme. Only modest regional differences were observed (miR\u2010122\u20105p ICC 0.864; miR\u2010122\u20103p ICC 0.849). Importantly, intra\u2010individual miR\u2010122 levels were stable over time (CV 10.7% and 16.5%, respectively), even under physiological perturbations such as fasting and postprandial states. Our findings suggest that previously reported miR\u2010122 variability may largely reflect analytical factors, including normalization strategies, rather than biological instability. miR\u2010122 is a robust biomarker for liver injury when appropriate analytical approaches and individual baseline levels are considered, supporting its application in clinical pharmacology and drug development.\n                  <\/jats:p>","DOI":"10.1002\/bcp.70836","type":"journal-article","created":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T04:20:39Z","timestamp":1789791639000},"source":"Crossref","is-referenced-by-count":0,"title":["Re\u2010evaluating variability of circulating miR\u2010122 in healthy volunteers: Implications for biomarker qualification in drug\u2010induced liver injury"],"prefix":"10.1002","author":[{"given":"Irum","family":"Javid","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Therapeutics, Institute of Systems, Molecular and Integrative Biology University of Liverpool  Liverpool UK"},{"name":"Department of Biochemistry Sardar Bahadur Khan Women's University  Quetta Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3378-5524","authenticated-orcid":false,"given":"Giusy","family":"Russomanno","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, Institute of Systems, Molecular and Integrative Biology University of Liverpool  Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Evans","sequence":"additional","affiliation":[{"name":"Computational Biology Facility, Liverpool Shared Research Facilities University of Liverpool  Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guruprasad P.","family":"Aithal","sequence":"additional","affiliation":[{"name":"Nottingham Digestive Diseases Centre, Translational Medical Sciences, School of Medicine University of Nottingham  Nottingham UK"},{"name":"NIHR Nottingham Biomedical Research Centre, Nottingham University Hospitals NHS Trust and the University of Nottingham  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiva S.","family":"Forootan","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, Institute of Systems, Molecular and Integrative Biology University of Liverpool  Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Warren E.","family":"Glaab","sequence":"additional","affiliation":[{"name":"Merck &amp; Co., Inc.  Rahway New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Hackl","sequence":"additional","affiliation":[{"name":"TAmiRNA GmbH  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joely","family":"Irlam","sequence":"additional","affiliation":[{"name":"Private consultant  Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kseniya","family":"Khamina\u2010Kotisch","sequence":"additional","affiliation":[{"name":"TAmiRNA GmbH  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha","family":"Korver","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, Institute of Systems, Molecular and Integrative Biology University of Liverpool  Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerd A.","family":"Kullak\u2010Ublick","sequence":"additional","affiliation":[{"name":"TransHeps AG  Weiningen Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophia L.","family":"Samodelov","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology and Toxicology, University Hospital Z\u00fcrich University of Z\u00fcrich  Z\u00fcrich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher E.","family":"Goldring","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, Institute of Systems, Molecular and Integrative Biology University of Liverpool  Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23116049"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/14737159.2018.1415145"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.4161\/rna.1.2.1066"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0045652"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.29802"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23031226"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010014\u20100550\u20108"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.29865"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.24538"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1412608111"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0220406"}],"container-title":["British Journal of Clinical Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bcp.70836","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bcp.70836","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bcp.70836","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T04:20:40Z","timestamp":1789791640000},"score":1,"resource":{"primary":{"URL":"https:\/\/bpspubs.onlinelibrary.wiley.com\/doi\/10.1002\/bcp.70836"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,18]]},"references-count":11,"alternative-id":["10.1002\/bcp.70836"],"URL":"https:\/\/doi.org\/10.1002\/bcp.70836","archive":["Portico"],"relation":{},"ISSN":["0306-5251","1365-2125"],"issn-type":[{"value":"0306-5251","type":"print"},{"value":"1365-2125","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,18]]},"article-number":"bcp.70836"}}