{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:34:34Z","timestamp":1788870874833,"version":"build-2803163510"},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"BOF","award":["BOF B\/09042\/02"],"award-info":[{"award-number":["BOF B\/09042\/02"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biopharm &amp; Drug Disp"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The microsomal protein per gram of liver (MPPGL) is an important scaling factor in the\n                    <jats:italic>in vitro\u2013in vivo<\/jats:italic>\n                    extrapolation of metabolic data obtained in liver microsomes. This study aimed to determine the MPPGL in four biliary atresia patients (0.6\u20131.6\u2009years old) undergoing liver transplantation, as it is known that the MPPGL is affected by age and possibly by liver disease. Due to the presence of bilirubin in the homogenates and microsomes, the NADPH\u2010cytochrome reductase activity was used to determine the recovery factor, rather than methods using the dithionite difference spectrum. A mean value of 18.73 (\u00b1 2.82) mg\/g (geometric mean\u2009\u00b1\u2009SD,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20094) was observed, which is lower than the expected MPPGL based on the age of the patients (26.60\u2009\u00b1\u20090.40\u2009mg\/g). This suggests a decreased amount of microsomal protein in the livers of biliary atresia patients. Moreover, no differences in MPPGL between different zones of the liver could be detected. Copyright \u00a9 2014 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/bdd.1895","type":"journal-article","created":{"date-parts":[[2014,3,18]],"date-time":"2014-03-18T07:41:18Z","timestamp":1395128478000},"page":"308-312","source":"Crossref","is-referenced-by-count":11,"title":["Microsomal protein per gram of liver (MPPGL) in paediatric biliary atresia patients"],"prefix":"10.1002","volume":"35","author":[{"given":"Lies","family":"De Bock","sequence":"first","affiliation":[{"name":"Laboratory of Medical Biochemistry and Clinical Analysis, Faculty of Pharmaceutical Sciences Ghent University Harelbekestraat 72 9000 Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koen","family":"Boussery","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Biochemistry and Clinical Analysis, Faculty of Pharmaceutical Sciences Ghent University Harelbekestraat 72 9000 Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth","family":"De Bruyne","sequence":"additional","affiliation":[{"name":"Ghent University Hospital Paediatric Gastroenterology Department De Pintelaan 185 9000 Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myriam","family":"Van Winckel","sequence":"additional","affiliation":[{"name":"Ghent University Hospital Paediatric Gastroenterology Department De Pintelaan 185 9000 Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Stephenne","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Catholique de Louvain Cliniques St Luc, D\u00e9partement de P\u00e9diatrie, Institut de Recherche Exp\u00e9rimentale et Clinique (PEDI unit) Av Hippocrate 10 1200 Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etienne","family":"Sokal","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Catholique de Louvain Cliniques St Luc, D\u00e9partement de P\u00e9diatrie, Institut de Recherche Exp\u00e9rimentale et Clinique (PEDI unit) Av Hippocrate 10 1200 Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Van Bocxlaer","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Biochemistry and Clinical Analysis, Faculty of Pharmaceutical Sciences Ghent University Harelbekestraat 72 9000 Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.108.021311"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920007779315053"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095\u2010006\u20109531\u20102"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102125.2003.01881.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088\u2010200645090\u201000005"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.110.037192"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.111.043752"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.2165\/11318160\u2010000000000\u201000000"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006\u20102952(87)90300\u20105"},{"key":"e_1_2_6_11_1","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/S0090-9556(25)05782-4","article-title":"Cytochrome\u2010P\u2010450 measurement in rat\u2010liver homogenate and microsomes \u2013 its use for correction of microsomal losses incurred by differential centrifugation","volume":"3","author":"Joly JG","year":"1975","journal-title":"Drug Metab Dispos"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006\u20102952(90)90052\u2010M"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0b013e318181b21c"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.112.048504"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1976.9999"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.121"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(20)82244-3"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003\u20102697(76)90114\u20107"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(78)52009-0"}],"container-title":["Biopharmaceutics &amp; 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