{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T14:06:36Z","timestamp":1770818796644,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,1,26]],"date-time":"2016-01-26T00:00:00Z","timestamp":1453766400000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["P303\/120850"],"award-info":[{"award-number":["P303\/120850"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["P303\/13-31118P"],"award-info":[{"award-number":["P303\/13-31118P"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007397","name":"Univerzita Karlova","doi-asserted-by":"publisher","award":["SVV\/2015\/260-185"],"award-info":[{"award-number":["SVV\/2015\/260-185"]}],"id":[{"id":"10.13039\/100007397","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biopharm &amp; Drug Disp"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Zidovudine (AZT) is one of the most frequently used antiretroviral drugs in prevention of perinatal transmission of HIV. However, safety concerns on AZT use in pregnancy still persist as severe side effects are associated with AZT exposure in children. In our study we aimed to contribute to current knowledge on AZT transplacental transport and to evaluate potential involvement of the main human drug efflux ATP\u2010binding cassette (ABC) transporters, p\u2010glycoprotein (ABCB1), breast cancer resistance protein (ABCG2) and multidrug resistance\u2010associated proteins 2 and 5 (ABCC2 and ABCC5) in the disposition of AZT between mother and fetus. In order to elucidate this issue we investigated the effect of selected ABC transporters on AZT transepithelial transport across MDCKII cell monolayers. In addition we used the in situ method of dually perfused rat term placenta to further study the role of ABC transporters in AZT transplacental transport. In vitro studies revealed significant effect of ABCB1 and ABCG2 on AZT transport which was subsequently confirmed also on organ level. Lamivudine, an antiretroviral agent commonly co\u2010administered with AZT, did not affect ABC transporter\u2010mediated AZT transfer. Copyright \u00a9 2016 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/bdd.1993","type":"journal-article","created":{"date-parts":[[2015,9,21]],"date-time":"2015-09-21T19:07:15Z","timestamp":1442862435000},"page":"28-38","source":"Crossref","is-referenced-by-count":25,"title":["Role of ABCB1, ABCG2, ABCC2 and ABCC5 transporters in placental passage of zidovudine"],"prefix":"10.1002","volume":"37","author":[{"given":"Zuzana","family":"Neumanova","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology Charles University in Prague, Faculty of Pharmacy in Hradec Kralove  Hradec Kralove Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lukas","family":"Cerveny","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Charles University in Prague, Faculty of Pharmacy in Hradec Kralove  Hradec Kralove Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Ceckova","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Charles University in Prague, Faculty of Pharmacy in Hradec Kralove  Hradec Kralove Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frantisek","family":"Staud","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology Charles University in Prague, Faculty of Pharmacy in Hradec Kralove  Hradec Kralove Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,1,26]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.283.9.1175"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199411033311801"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0000000000000212"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/507645"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-200410210-00005"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-200308150-00006"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ260"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/0091270002239821"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3109\/1061186X.2012.716847"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3109\/03602532.2010.512293"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)62230-4"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.104.003335"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2006.01.007"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.11.4332-4336.2004"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1086\/315152"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1086\/314431"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.AOG.0000178160.38042.04"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/161.2.203"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-0997(1997)5:4<310::AID-IDOG9>3.0.CO;2-#"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000441-199001000-00005"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2011.04.007"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.106.014274"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.63.1.65"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.21744"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.107.017723"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/135965350400900403"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3181\/0802-RM-59"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2004.05.052"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl474"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2125.2007.02944.x"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.106.012765"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528-1167.2006.00453.x"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0000000000000112"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.301.3.797"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2008.08.032"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.106.105023"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/163.2.226"},{"issue":"12","key":"e_1_2_9_39_1","doi-asserted-by":"crossref","first-page":"5748","DOI":"10.1016\/S0021-9258(18)45638-4","article-title":"3'\u2010azido\u20103'\u2010deoxythymidine. An unusual nucleoside analogue that permeates the membrane of human erythrocytes and lymphocytes by nonfacilitated diffusion","volume":"262","author":"Zimmerman TP","year":"1987","journal-title":"J Biol Chem"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-008-9564-9"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.300.3.918"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3028"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2015.07.070"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.32.8.813"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1080\/00498250500354493"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.102.037580"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2009.10.001"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.3.991-996.2003"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2007.07.004"},{"key":"e_1_2_9_50_1","first-page":"1","article-title":"Regulation of drug transporter expression and function in the placenta","author":"Staud F","year":"2015","journal-title":"Expert Opin Drug Metab Toxicol"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40264-015-0299-3"}],"container-title":["Biopharmaceutics &amp; Drug Disposition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fbdd.1993","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bdd.1993","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T18:08:27Z","timestamp":1696615707000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bdd.1993"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1]]},"references-count":50,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,1]]}},"alternative-id":["10.1002\/bdd.1993"],"URL":"https:\/\/doi.org\/10.1002\/bdd.1993","archive":["Portico"],"relation":{},"ISSN":["0142-2782","1099-081X"],"issn-type":[{"value":"0142-2782","type":"print"},{"value":"1099-081X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1]]}}}