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The results of the single\u2010pass intestinal perfusion indicated that the jejunum and ileum are the main absorption sites, and there was carrier saturation. In addition, fucoidan sulfate at 1\u00a0mg\/ml had no inhibitory effect on Caco\u20102 cell proliferation. Studies on the transmembrane transport mechanism showed that fucoidan can be absorbed because the apparent permeability coefficient of the drugs (P<jats:sub>app<\/jats:sub>) A\u00a0\u2192\u00a0B was 3.78\u00a0+\u00a00.03 \u00d710<jats:sup>\u22126<\/jats:sup> and that of B\u00a0\u2192\u00a0A was 1.42\u00a0+\u00a00.19 \u00d710<jats:sup>\u22126<\/jats:sup>. The peak absorption of fucoidan occurred at 120\u00a0min after administration; moreover, the higher the concentration used, the worse the absorption was, suggesting the saturation of transport carriers. The absorption was temperature dependent: the absorption at 37\u00b0C was much better than that at 4\u00b0C. Further, the absorption of fucoidan sulfate might rely on clathrin endocytosis as chlorpromazine (10\u00a0\u03bcg\/ml) significantly inhibited it.<\/jats:p>","DOI":"10.1002\/bdd.2137","type":"journal-article","created":{"date-parts":[[2018,6,15]],"date-time":"2018-06-15T02:37:39Z","timestamp":1529030259000},"page":"298-307","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Use of fluorescein isothiocyanate isomer I to study the mechanism of intestinal absorption of fucoidan sulfate <i>in vivo<\/i> and <i>in vitro<\/i>"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3744-6351","authenticated-orcid":false,"given":"E","family":"Zhang","sequence":"first","affiliation":[{"name":"Marine College Shandong University  Weihai China"},{"name":"Weihai International Biotechnology Research and Development Centre Shandong University  Weihai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fulong","family":"Chu","sequence":"additional","affiliation":[{"name":"Marine College Shandong University  Weihai China"},{"name":"Weihai International Biotechnology Research and Development Centre Shandong University  Weihai China"},{"name":"Food and Drug Administration of Beijing Haidian District  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lixu","family":"Xu","sequence":"additional","affiliation":[{"name":"Marine College Shandong University  Weihai China"},{"name":"Weihai International Biotechnology Research and Development Centre Shandong University  Weihai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Liang","sequence":"additional","affiliation":[{"name":"Marine College Shandong University  Weihai China"},{"name":"Weihai International Biotechnology Research and Development Centre Shandong University  Weihai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuliang","family":"Song","sequence":"additional","affiliation":[{"name":"Marine College Shandong University  Weihai China"},{"name":"Weihai International Biotechnology Research and Development Centre Shandong University  Weihai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aiguo","family":"Ji","sequence":"additional","affiliation":[{"name":"Marine College Shandong University  Weihai China"},{"name":"Weihai International Biotechnology Research and Development Centre Shandong University  Weihai China"},{"name":"School of Pharmaceutical Sciences Shandong University  Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,7,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2012.06.033"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2008.10.008"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mex.2016.03.010"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6215(00)81824-8"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.6b00821"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2016.01.006"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mex.2015.03.006"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.6b00401"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1358\/mf.2005.27.10.948919"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2006.08.010"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.52.1091"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2016.04.014"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018446723616"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/03639040701833948"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3151"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19960129"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2015.05.026"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/md13010048"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwn085"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2010.05.010"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2017.09.045"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.3109\/00498254.2015.1121553"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1012102522787"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2016.03.008"}],"container-title":["Biopharmaceutics &amp; 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