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Here, we focused on the barrier function of the small intestine, especially in tight junctions, and compared it with that of the colon. Dextran sulfate sodium (DSS) was used to induce ulcerative colitis in rats in order to evaluate the function of the paracellular barrier in the jejunum, ileum, and colon. An <jats:italic>in vitro<\/jats:italic> diffusion chamber method was used to evaluate membrane resistance, which is an index of tight junction function and mucosal permeability, using 6\u2010carboxyfluorescein (6\u2010CF), a paracellular marker. In the jejunum and colon, with decrease of membrane resistance in the DSS group, mucosal permeability increased, whereas no marked difference was observed in the ileum. In the <jats:italic>in situ<\/jats:italic> closed\u2010loop method, absorption of 6\u2010CF from the jejunum was higher than that from the ileum. Immunohistochemical staining of claudin\u20104 showed heterogeneous attenuation of claudin\u20104 in the jejunum. Pharmacokinetic parameters were calculated from the blood concentration after intravenous injection and oral administration of 6\u2010CF. In the DSS group, there was a delay in the elimination phase, suggesting a decrease in renal function, and an increase in maximum blood concentration, associated with an increased absorption rate constant. The increased absorption and decreased renal function due to decreased paracellular barrier function in the small intestine and colon may cause fluctuations in drug efficacy and side effects.<\/jats:p>","DOI":"10.1002\/bdd.2220","type":"journal-article","created":{"date-parts":[[2020,2,11]],"date-time":"2020-02-11T09:18:20Z","timestamp":1581412700000},"page":"91-100","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Increased membrane permeation and blood concentration of 6\u2010carboxyfluorescein associated with dysfunction of paracellular route barrier in the small intestine of ulcerative colitis model rats"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0597-5985","authenticated-orcid":false,"given":"Maho","family":"Kumagai","sequence":"first","affiliation":[{"name":"Drug Absorption and Pharmacokinetics Tohoku Medical and Pharmaceutical University  Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Ishii","sequence":"additional","affiliation":[{"name":"Drug Absorption and Pharmacokinetics Tohoku Medical and Pharmaceutical University  Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5328-8843","authenticated-orcid":false,"given":"Kaori","family":"Morimoto","sequence":"additional","affiliation":[{"name":"Drug Absorption and Pharmacokinetics Tohoku Medical and Pharmaceutical University  Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikio","family":"Tomita","sequence":"additional","affiliation":[{"name":"Drug Absorption and Pharmacokinetics Tohoku Medical and Pharmaceutical University  Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,2,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/apm.12814"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.20018"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2015.05.006"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01071972"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142735.im1525s104"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2017.05.056"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2014.01.028"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018943613122"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600581006"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.disamonth.2019.02.004"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v17.i17.2211"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00019.2012"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-017-2227-y"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xphs.2017.04.046"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1254\/jphs.13141FP"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2013.12.019"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2017.11.032"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.5b00769"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2017.03.007"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.2008.05405.x"},{"issue":"9","key":"e_1_2_9_22_1","first-page":"1139","article-title":"Inflammatory processes have differential effects on claudins 2, 3 and 4 incolonic epithelial cells. 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