{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T23:44:29Z","timestamp":1789775069139,"version":"4.0.1"},"reference-count":48,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,5,6]],"date-time":"2021-05-06T00:00:00Z","timestamp":1620259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003010","name":"Ege University","doi-asserted-by":"publisher","award":["17 NBE 007"],"award-info":[{"award-number":["17 NBE 007"]}],"id":[{"id":"10.13039\/501100003010","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biopharm &amp; Drug Disp"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Early detection of the site of infection non\u2010invasively with radiolabeled molecules is important for the success of treatment. Technetium\u201099m labeled antibiotics have the potential to discriminate between bacterial infection and sterile inflammation. Sultamicillin is the tosylate salt of the double ester of sulbactam plus ampicillin. In this study, sultamicillin was labeled with <jats:sup>99m<\/jats:sup>Tc according to the stannous chloride method. Quality control studies of radiolabeled sultamicillin were performed by radiochromatographic methods. <jats:italic>In vitro<\/jats:italic> binding assays were performed in live and heat\u2010killed gram\u2010positive <jats:italic>Staphylococcus aureus<\/jats:italic> and gram\u2010negative <jats:italic>Escherichia coli<\/jats:italic> strains. The radiolabeling yield of <jats:sup>99m<\/jats:sup>Tc\u2010sultamicillin was determined as 97.8%\u00a0\u00b1\u00a03.1% (n\u00a0=\u00a05). The maximum bacterial uptake of <jats:sup>99m<\/jats:sup>Tc\u2010sultamicillin was 80.7%\u00a0\u00b1\u00a011.00% at 4\u00a0h for living <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> and 93.2%\u00a0\u00b1\u00a04.40% at 2\u00a0h for <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic>. Bacterial uptake study results show that sultamicillin has the potential to be a nuclear imaging agent, especially in infections caused by gram\u2010negative <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> and gram\u2010positive <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/bdd.2281","type":"journal-article","created":{"date-parts":[[2021,4,27]],"date-time":"2021-04-27T05:45:35Z","timestamp":1619502335000},"page":"285-293","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>In vitro<\/i> evaluation of <sup>99m<\/sup>Tc\u2010sultamicillin for infection imaging"],"prefix":"10.1002","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0783-8557","authenticated-orcid":false,"given":"Kubra","family":"Durkan","sequence":"first","affiliation":[{"name":"Nuclear Applications Ege University Institute of Nuclear Sciences  Bornova Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayca","family":"Tuncel","sequence":"additional","affiliation":[{"name":"Nuclear Applications Ege University Institute of Nuclear Sciences  Bornova Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma","family":"Yurt","sequence":"additional","affiliation":[{"name":"Nuclear Applications Ege University Institute of Nuclear Sciences  Bornova Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,5,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/14732300020300S103"},{"issue":"4","key":"e_1_2_7_3_1","first-page":"272","article-title":"Binding of ciprofloxacin labelled with technetium Tc 99m versus 99mTc\u2010pertechnetate to a live and killed equine isolate of Escherichia coli","volume":"69","author":"Alexander K.","year":"2005","journal-title":"Canadian Journal of Veterinary Research = Revue Canadienne de Recherche Veterinaire"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40336-016-0185-8"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01850.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1516-89132002000500005"},{"key":"e_1_2_7_7_1","volume-title":"The European Committee on Antimicrobial Susceptibility Testing. Routine and extended internal quality control for MIC determination and disk diffusion as recommended by EUCAST","author":"EUCAST","year":"2017"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10967-013-2523-x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.semnuclmed.2008.08.002"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12010-014-1075-z"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.2310\/7290.2011.00051"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12149-010-0417-3"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2008.02.004"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10967-013-2418-x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10967-010-0826-8"},{"key":"e_1_2_7_16_1","first-page":"15","article-title":"99mTc\u2010Labeled ceftazidime and biological evaluation in experimental animals for detection of bacterial infection","volume":"4","author":"Mohamed S.","year":"2014","journal-title":"American Journal of Biochemistry"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apradiso.2010.04.031"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10967-006-6754-y"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10967-006-6786-3"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlcr.1619"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10156-008-0634-Z"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20225808"},{"issue":"3","key":"e_1_2_7_23_1","first-page":"2745","article-title":"Synthesis, radiolabelling and biological characteristics of a bombesin peptide analog as a tumor imaging agent","volume":"23","author":"Okarvi S. M.","year":"2003","journal-title":"Anticancer Research"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00259-004-1722-7"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40336-013-0041-z"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10967-010-0470-3"},{"issue":"2","key":"e_1_2_7_27_1","first-page":"91","article-title":"Studying the effects of several heat\u2010inactivated bacteria on colon and breast cancer cells","volume":"8","author":"Rabiei P.","year":"2019","journal-title":"Molecular Biology Research Communications"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200767130-00003"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2009.08.001"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10967-006-0087-8"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.semnuclmed.2017.10.003"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1134\/S1066362213050159"},{"key":"e_1_2_7_33_1","first-page":"920","article-title":"Inability of 99mTc\u2010ciprofloxacin scintigraphy to discriminate between septic and sterile osteoarticular diseases","volume":"44","author":"Sarda\u2010Mantel L.","year":"2003","journal-title":"Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine"},{"issue":"2","key":"e_1_2_7_34_1","first-page":"73","article-title":"Synthesis and experimental study of norfloxacin labeled with technecium\u201099m as a potential agent for infection imaging","volume":"23","author":"Sazonova S. I.","year":"2015","journal-title":"Iranian Journal of Nuclear Medicine"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apradiso.2010.05.014"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13139-011-0106-8"},{"issue":"8","key":"e_1_2_7_37_1","first-page":"1","article-title":"Synthesis, characterization, biodistribution and scintigraphic evaluation of technetium\u201099m (99mTc) labeled pazufloxacin (PZN): A novel infection radiotracer","volume":"8","author":"Shah S. Q.","year":"2015","journal-title":"Nuclear Medicine and Radiation Therapy"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arabjc.2015.04.003"},{"issue":"12","key":"e_1_2_7_39_1","first-page":"2088","article-title":"Synthesis and comparison of 99mTc\u2010enrofloxacin and 99mTc\u2010ciprofloxacin","volume":"45","author":"Siaens R. H.","year":"2004","journal-title":"Journal of Nuclear Medicine"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00259-008-0762-9"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12149-011-0521-z"},{"key":"e_1_2_7_42_1","first-page":"1","article-title":"The need of shared diagnostic protocols","volume":"58","author":"Signore A.","year":"2014","journal-title":"The Quarterly Journal of Nuclear Medicine and Molecular Imaging"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a000414"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2019.04.002"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-8051(02)00348-7"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10967-017-5691-2"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)90407-9"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/cbdd.13171"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10967-007-7111-5"}],"container-title":["Biopharmaceutics &amp; Drug Disposition"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bdd.2281","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bdd.2281","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bdd.2281","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T20:01:16Z","timestamp":1693425676000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bdd.2281"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,6]]},"references-count":48,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,6]]}},"alternative-id":["10.1002\/bdd.2281"],"URL":"https:\/\/doi.org\/10.1002\/bdd.2281","archive":["Portico"],"relation":{},"ISSN":["0142-2782","1099-081X"],"issn-type":[{"value":"0142-2782","type":"print"},{"value":"1099-081X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,6]]},"assertion":[{"value":"2020-12-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}