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The intestinal absorption of Daclatasvir was assessed in four segments: duodenum, jejunum, ileum, and colon. The effect of co\u2010perfusion with omeprazole was monitored through the tested anatomical sites. The effect of piperine, a P\u2010glycoprotein (P\u2010gp) inhibitor on Daclatasvir absorption from jejunum and ileum was tested. The results showed that Daclatasvir was incompletely absorbed from the rabbit small and large intestine. The absorptive clearance per unit length (PeA\/L) was site dependent and was ranked as colon\u00a0&gt;\u00a0duodenum\u00a0&gt;\u00a0jejunum\u00a0&gt;\u00a0ileum. This rank is the opposite of the rank of P\u2010gp intestinal content suggesting possible influence for P\u2010gp. Co\u2010perfusion with omeprazole increased PeA\/L and this was evidenced also with reduced the L95% of Daclatasvir from both small and large intestinal segments. Significant enhancement in Daclatasvir absorption through jejunum and ileum was shown in presence of piperine. Daclatasvir showed site dependent intestinal absorption in a manner suggesting its affection by P\u2010gp efflux. This effect was inhibited by piperine. Co\u2010administration of Daclatasvir with omeprazole can enhance intestinal absorption a phenomenon which requires extension to human pharmacokinetic investigation.<\/jats:p>","DOI":"10.1002\/bdd.2308","type":"journal-article","created":{"date-parts":[[2022,1,8]],"date-time":"2022-01-08T01:38:34Z","timestamp":1641605914000},"page":"33-44","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Influence of piperine and omeprazole on the regional absorption of Daclatasvir from rabbit intestine"],"prefix":"10.1002","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8095-882X","authenticated-orcid":false,"given":"Shimaa M.","family":"Ashmawy","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Technology College of Pharmacy University of Tanta  Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dina A.","family":"Eltahan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology College of Pharmacy University of Tanta  Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed A.","family":"Osman","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology College of Pharmacy University of Tanta  Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ebtessam A.","family":"Essa","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology College of Pharmacy University of Tanta  Tanta Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,1,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/6535816"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-5193(81)90306-4"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2125.1994.tb04310.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/clpt.2010.219"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jphp.13036"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(13)60796-5"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.2165\/00002018-200629090-00002"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2018.09.022"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.2007.04691.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.24122"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600581006"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2009.142"},{"issue":"2","key":"e_1_2_9_14_1","first-page":"730","article-title":"Inhibition by omeprazole of proguanil metabolism: Mechanism of the interaction in vitro and prediction of in vivo results from the in vitro experiments","volume":"280","author":"Funck\u2010Brentano C.","year":"1997","journal-title":"Journal of Pharmacology and Experimental Therapeutics"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40262-017-0624-3"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2018.11.008"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2008.02.026"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bmy079"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3841.2010.01542.x"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40265-016-0632-x"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-014-1336-0"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-5173(80)90140-4"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/MEG.0000000000000152"},{"issue":"2","key":"e_1_2_9_24_1","first-page":"91","article-title":"Mucosal penetration enhancers for facilitation of peptide and protein drug absorption","volume":"8","author":"Lee V. 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