{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T17:03:36Z","timestamp":1786986216326,"version":"build-2736575974"},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,6,5]],"date-time":"2023-06-05T00:00:00Z","timestamp":1685923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100018810","name":"Over-the-counter Drug Self-Medication Promotion Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018810","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Biopharm &amp; Drug Disp"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\u03b1\u2010Defensin 5 is known to be secreted by Paneth cells in the small intestine and plays an important role in eliminating pathogenic microorganisms. It has been reported that a decrease in \u03b1\u2010defensin 5 level in the human small intestine is a risk of inflammatory bowel disease (IBD). Furthermore, P\u2010glycoprotein (P\u2010gp), a member of the ATP\u2010binding cassette transporter superfamily, encoded by the ABCB1\/MDR1 gene, plays an important role in the front line of host defense by protecting the gastrointestinal barrier from xenobiotic accumulation and may contribute to the development and persistence of IBD. Therefore, we examined the relationship between \u03b1\u2010defensin 5 and the expression and function of P\u2010gp using a human gastrointestinal model cell line (Caco\u20102). We found that <jats:italic>MDR1<\/jats:italic> mRNA and P\u2010gp protein level were increased in Caco\u20102 cells as well as \u03b1\u2010defensin 5 secretion corresponded with the duration of cell culture. Exposure to \u03b1\u2010defensin 5 peptide and recombinant tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) significantly increased the expression and function P\u2010gp. The mRNA levels of interleukin (<jats:italic>IL<\/jats:italic>)<jats:italic>\u2010<\/jats:italic><jats:italic>8<\/jats:italic>, <jats:italic>IL\u20106<\/jats:italic>, <jats:italic>TNF\u2010\u03b1<\/jats:italic>, <jats:italic>IL\u20101\u03b2<\/jats:italic>, and <jats:italic>IL\u20102<\/jats:italic> were also increased following exposure to TNF\u2010\u03b1, similar to \u03b1\u2010defensin 5 treatment. These results suggest that \u03b1\u2010defensin 5 regulates P\u2010gp expression and function by increasing TNF\u2010\u03b1 expression in Caco\u20102 cells.<\/jats:p>","DOI":"10.1002\/bdd.2367","type":"journal-article","created":{"date-parts":[[2023,6,7]],"date-time":"2023-06-07T08:22:36Z","timestamp":1686126156000},"page":"358-364","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Association between \u03b1\u2010defensin 5 and the expression and function of P\u2010glycoprotein in differentiated intestinal Caco\u20102 cells"],"prefix":"10.1002","volume":"44","author":[{"given":"Genki","family":"Yasuda","sequence":"first","affiliation":[{"name":"Laboratory of Clinical Pharmaceutics and Therapeutics Faculty of Pharmaceutical Sciences Hokkaido University  Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsuhito","family":"Kubota","sequence":"additional","affiliation":[{"name":"Laboratory 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