{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T11:13:23Z","timestamp":1774523603786,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,5,30]],"date-time":"2018-05-30T00:00:00Z","timestamp":1527638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001742","name":"United States-Israel Binational Science Foundation","doi-asserted-by":"publisher","award":["0374352"],"award-info":[{"award-number":["0374352"]}],"id":[{"id":"10.13039\/501100001742","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003977","name":"Israel Science Foundation","doi-asserted-by":"publisher","award":["0394193"],"award-info":[{"award-number":["0394193"]}],"id":[{"id":"10.13039\/501100003977","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2018,7,17]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Hyperglycemia\u2010related oxidative stress and hypoxia are important mechanisms responsible for diabetes\u2010induced embryopathy and other complications. High sucrose low copper diet (HSD), but not regular diet (RD), induces type 2 diabetes in the inbred Cohen diabetic sensitive (CDs) rats but not in the Sabra control rats. We recently demonstrated long\u2010term changes of DNA methylation and gene expression in various groups of genes, including genes involved in oxidant\u2010antioxidant activity in the liver of 2\u20134\u2010week\u2010old CDs offspring of diabetic dams. We now studied the postnatal effects of diabetes and\/or HSD on several liver metabolic parameters in these offspring.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>we studied lipid peroxidation, activity of the antioxidants enzymes superoxide dismutase (SOD) and Catalase (CAT). By immunohistochemistry: protein oxidation by nitrotyrosine staining, hypoxia inducing factor1\u03b1 (HIF1\u03b1), apoptosis [caspase 3, bcl\u20102\u2010like protein (BAX)], proliferation [proliferating cell nuclear antigen (PCNA)] and NF\u2010\u03baB.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the Sabra rats fed HSD only few, early and transitional changes were observed in lipid peroxidation, SOD and CAT activity. In the CDs fed HSD more significant changes in lipid and protein oxidation, HIF1\u03b1, apoptosis and proliferation were observed, persisting for longer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The changes in the Sabra rats HSD were attributed to the pro\u2010oxidant effects of the diet and those in the diabetic CDs to the HSD and maternal diabetes. In light of the DNA methylation changes in the liver of the CDs HSD, we presume that changes in gene expression are responsible for our findings, and that similar changes may lead to the metabolic syndrome at adulthood.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdr2.1341","type":"journal-article","created":{"date-parts":[[2018,5,31]],"date-time":"2018-05-31T05:51:31Z","timestamp":1527745891000},"page":"1001-1015","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["High sucrose low copper diet in pregnant diabetic rats induces transient oxidative stress, hypoxia, and apoptosis in the offspring's liver"],"prefix":"10.1002","volume":"110","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2216-6899","authenticated-orcid":false,"given":"Zivanit","family":"Ergaz","sequence":"first","affiliation":[{"name":"Neonatology, Hadassah Hebrew University Medical Center Jerusalem Israel"},{"name":"Laboratory of Teratology, Department of Medical Neurobiology Hebrew University Hadassah Medical School Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liza","family":"Weinstein\u2010Fudim","sequence":"additional","affiliation":[{"name":"Laboratory of Teratology, Department of Medical Neurobiology Hebrew University Hadassah Medical School Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7268-3512","authenticated-orcid":false,"given":"Asher","family":"Ornoy","sequence":"additional","affiliation":[{"name":"Laboratory of Teratology, Department of Medical Neurobiology Hebrew University Hadassah Medical School Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,5,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2015-10626"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20080413"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0174030"},{"key":"e_1_2_7_6_1","volume-title":"The Cohen Diabetic Rat","author":"Cohen A. 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