{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T09:27:35Z","timestamp":1762507655145,"version":"3.40.5"},"reference-count":82,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,2,21]],"date-time":"2019-02-21T00:00:00Z","timestamp":1550707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000027","name":"National Institute on Alcohol Abuse and Alcoholism","doi-asserted-by":"publisher","award":["F31AA024964","F32AA026770","R01AA023426"],"award-info":[{"award-number":["F31AA024964","F32AA026770","R01AA023426"]}],"id":[{"id":"10.13039\/100000027","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2019,7,15]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>A cardinal feature of prenatal ethanol exposure is CNS damage, resulting in a continuum of neurological and behavioral impairments that are described by the term fetal alcohol spectrum disorders (FASD). FASDs are variable and depend on several factors, including the amount, timing, and duration of prenatal ethanol exposure. To enhance interventions for CNS dysfunction, it is necessary to identify ethanol\u2010sensitive neuronal populations and expand the understanding of factors that modify ethanol teratogenesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>To investigate the susceptibility of different neuronal subtypes, we exposed transgenic zebrafish (<jats:styled-content style=\"fixed-case\"><jats:italic>Danio rerio<\/jats:italic><\/jats:styled-content>) to several ethanol concentrations (0.25, 0.5, 1.0, 1.5, or 2.0%), at different hours post fertilization (hpf; 0, 6, or 24 hpf), for various durations (0\u201324, 0\u201348, 4\u201324, 6\u201324, 6\u201348,or 24\u201348 hpf). Following exposure, embryo survival rates were determined, and CNS neurogenesis, differentiation, and patterning were assessed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Embryo survival rates decrease as ethanol concentrations increase and drastically decline when exposed from 0\u201324 hpf compared to 4\u201324 hpf. Abnormal tangential migration of facial motor neurons is observed in <jats:italic>isl1:gfp<\/jats:italic> embryos exposed to ethanol concentrations as low as 0.25%, and the formation of IVth ventricle heterotopias are revealed by embryos exposed to \u22651.0% ethanol. Whereas, expression of <jats:italic>olig2:dsred<\/jats:italic> and <jats:italic>ptf1a:gfp<\/jats:italic> in the cerebellum and spinal cord are largely unaffected. While levels of <jats:italic>etv4<\/jats:italic> mRNA are overtly resistant to ethanol, we observe significant reductions in <jats:italic>ptch2<\/jats:italic> mRNA levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These data show differentially sensitive CNS neuron subpopulations with susceptibility to low levels of ethanol. In addition, these data reveal the formation of ethanol\u2010induced hindbrain heterotopias.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdr2.1477","type":"journal-article","created":{"date-parts":[[2019,2,22]],"date-time":"2019-02-22T06:25:57Z","timestamp":1550816757000},"page":"700-713","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Differentially sensitive neuronal subpopulations in the central nervous system and the formation of hindbrain heterotopias in ethanol\u2010exposed zebrafish"],"prefix":"10.1002","volume":"111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4780-8173","authenticated-orcid":false,"given":"Desire M.","family":"Buckley","sequence":"first","affiliation":[{"name":"Department of Molecular Biosciences The University of Texas at Austin  Austin Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfire","family":"Sidik","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences The University of Texas at Austin  Austin Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ranjeet D.","family":"Kar","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences The University of Texas at Austin  Austin Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johann K.","family":"Eberhart","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences The University of Texas at Austin  Austin Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,2,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.162356199"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdra.20447"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/10826084.2014.912228"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.04.013"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/dneu.20761"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.1990.tb00455.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/tera.1420440203"},{"issue":"19","key":"e_1_2_6_9_1","first-page":"529","article-title":"Alcohol use and binge drinking among women of childbearing age: United States, 2006\u20132010","volume":"58","author":"CDC","year":"2012","journal-title":"Morbidity and Mortality Weekly Report"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394310-1.00007-2"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.9.2835"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000066748"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.11-02-00311.1991"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1139\/bcb-2017-0122"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.contraception.2011.07.013"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2017.00064"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00108.2017"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02075"},{"issue":"3","key":"e_1_2_6_19_1","first-page":"175","article-title":"Mechanisms of alcohol\u2010induced damage to the developing nervous system","volume":"25","author":"Goodlett C. 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