{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T23:01:10Z","timestamp":1791846070745,"version":"4.3.4"},"reference-count":37,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,3,19]],"date-time":"2019-03-19T00:00:00Z","timestamp":1552953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2019,7,15]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The need for earlier recognition of children at risk for neurobehavioral problems associated with prenatal ethanol exposure (PAE) has prompted investigations of biomarkers prognostic for altered fetal development. Here, we examined whether PAE alters the expression of angiogenesis\u2010related proteins and cytokines in human placenta in subjects from an Ethanol, Neurodevelopment, Infant and Child Health prospective cohort.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>PAE was ascertained by screening questionnaires, Time\u2010line Follow\u2010back interviews and a panel of ethanol biomarkers at two study visits. After delivery, placental tissue samples were collected for protein analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>No significant differences in the prevalence of substance use, demographic or medical characteristics were observed between the No PAE and PAE groups. PAE was associated with significant reductions in placental expression of VEGFR2 and annexin\u2010A4, while the levels of VEGFR1 and CCM\u20103 trended downward. A trend toward higher expression of the cytokines TNF\u2010\u03b1 and IL\u201013 was also observed in the PAE group. Receiver operating characteristic analyses of the data demonstrated a moderate\u2010to\u2010high degree of diagnostic accuracy for individual placental proteins. Combinations of proteins substantially increased their ability to differentiate between PAE and No PAE subjects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results establish the feasibility of harvesting placental tissue for protein analyses of PAE in a prospective manner. In addition, given the importance of vascular remodeling in both placenta and developing brain, the role of angiogenic and cytokine proteins in this process warrants further investigation for their utility for predicting alterations in brain development, as well as their mechanistic role in PAE\u2010induced pathology.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdr2.1488","type":"journal-article","created":{"date-parts":[[2019,3,20]],"date-time":"2019-03-20T06:45:11Z","timestamp":1553064311000},"page":"749-759","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["The association between prenatal alcohol exposure and protein expression in human placenta"],"prefix":"10.1002","volume":"111","author":[{"given":"Bradley D.","family":"Holbrook","sequence":"first","affiliation":[{"name":"Department of Obstetrics &amp; Gynecology, School of Medicine University of New Mexico  Albuquerque New Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9992-9551","authenticated-orcid":false,"given":"Suzy","family":"Davies","sequence":"additional","affiliation":[{"name":"Department of Neurosciences, School of Medicine University of New Mexico  Albuquerque New Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Cano","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Practice &amp; Administrative Sciences College of Pharmacy, University of New Mexico  Albuquerque New Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4349-8326","authenticated-orcid":false,"given":"Shikhar","family":"Shrestha","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Practice &amp; Administrative Sciences College of Pharmacy, University of New Mexico  Albuquerque New Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5410-3553","authenticated-orcid":false,"given":"Lauren L.","family":"Jantzie","sequence":"additional","affiliation":[{"name":"Department of Neurosciences, School of Medicine University of New Mexico  Albuquerque New Mexico"},{"name":"Department of Pediatrics, School of Medicine University of New Mexico  Albuquerque New Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William F.","family":"Rayburn","sequence":"additional","affiliation":[{"name":"Department of Obstetrics &amp; Gynecology, School of Medicine University of New Mexico  Albuquerque New Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9877-0211","authenticated-orcid":false,"given":"Ludmila N.","family":"Bakhireva","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Practice &amp; Administrative Sciences College of Pharmacy, University of New Mexico  Albuquerque New Mexico"},{"name":"Department of Family &amp; Community Medicine, School of Medicine University of New Mexico  Albuquerque New Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1726-1103","authenticated-orcid":false,"given":"Daniel D.","family":"Savage","sequence":"additional","affiliation":[{"name":"Department of Neurosciences, School of Medicine University of New Mexico  Albuquerque New Mexico"},{"name":"Department of Pediatrics, School of Medicine University of New Mexico  Albuquerque New Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0376-8716(84)90012-7"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0741-8329(00)00076-8"},{"issue":"2015","key":"e_1_2_6_4_1","article-title":"Ethanol, Neurodevelopment, Infant and Child Health (ENRICH) prospective cohort: Study design considerations","volume":"2","author":"Bakhireva L. 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