{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T17:34:29Z","timestamp":1774460069722,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2020,7,10]],"date-time":"2020-07-10T00:00:00Z","timestamp":1594339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100012043","name":"Kindai University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012043","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Although maternal MeHg\u2010exposure causes hydrocephalus in the offspring of mice, its pathogenesis has not been fully explained. In the present study, we examined the issue of how maternal MeHg\u2010exposure in mice affects ependymal ciliary movement in the offspring and whether the lateral ventricles in offspring show dilation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Pregnant mice were given drinking water containing 0, 10, or 20\u2009mg\/L MeHg, or a single dose of 2 mg\/kg MeHg. Brain slices were prepared from the offspring and the ependymal ciliary movement of ependymal cells in the third ventricle were observed by a high\u2010speed digital camera. The dilation of the lateral ventricles in the offspring was assessed by histological examination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The administration of MeHg in the drinking water of pregnant mice at levels of 10 mg\/L and 20\u2009mg\/L MeHg from GD10 to birth caused a significant decrease of ciliary beating frequency (CBF) in ependymal cells of the third ventricle in the weaned offspring. The ependymal ciliary movement of the weaned offspring was particularly sensitive in the case of the administration of MeHg at GD10. Moreover, there was a significant dilation of cross\u2010sectional areas of lateral ventricles in weaned offspring from the pregnant mice that had been administered MeHg. The CBF and the cross\u2010sectional areas of the lateral ventricles improved with time.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results suggest that the impairment of ependymal ciliary movement by maternal MeHg\u2010exposure contributes to the development of hydrocephalus in the offspring.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdr2.1750","type":"journal-article","created":{"date-parts":[[2020,7,10]],"date-time":"2020-07-10T12:23:47Z","timestamp":1594383827000},"page":"1253-1259","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Maternal exposure to methylmercury causes an impairment in ependymal cilia motility in the third ventricle and dilation of lateral ventricles in mice offspring"],"prefix":"10.1002","volume":"112","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5850-0273","authenticated-orcid":false,"given":"Teruki","family":"Hagiwara","sequence":"first","affiliation":[{"name":"Department of Life Science, School of Science &amp; Engineering Kindai University  Higashiosaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hajime","family":"Hagino","sequence":"additional","affiliation":[{"name":"Department of Science, Interdisciplinary Graduate School of Science and Engineering Kindai University  Higashiosaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaho","family":"Ueda","sequence":"additional","affiliation":[{"name":"Department of Science, Interdisciplinary Graduate School of Science and Engineering Kindai University  Higashiosaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mina","family":"Nakama","sequence":"additional","affiliation":[{"name":"Department of Science, Interdisciplinary Graduate School of Science and Engineering Kindai University  Higashiosaka Japan"},{"name":"Division of Clinical Genetics Gifu University Hospital  Gifu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Minami","sequence":"additional","affiliation":[{"name":"Department of Life Science, School of Science &amp; Engineering Kindai University  Higashiosaka Japan"},{"name":"Department of Science, Interdisciplinary Graduate School of Science and Engineering Kindai University  Higashiosaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03812-w"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtemb.2016.03.001"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00687784"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0892-0362(03)00007-2"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(13)70278-3"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.116.030791"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/tera.1420060204"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/11.6.715"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00316251"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/tera.1420410212"},{"issue":"3","key":"e_1_2_9_12_1","first-page":"227","article-title":"Behavioral and neuropathological effects of prenatal methylmercury exposure in mice","volume":"7","author":"Inouye M.","year":"1985","journal-title":"Neurobehavioral Toxicology and Teratology"},{"issue":"6","key":"e_1_2_9_13_1","first-page":"775","article-title":"Effects of the high methylmercury dose used in the collaborative behavioral teratology study on brain anatomy","volume":"7","author":"Kutscher C. 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