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However, there is still a need for understanding the embryonic morphological changes induced by ZIKV and when they occur. Here, chicken embryos were chosen as experimental model of ZIKV to evaluate virus\u2010associated morphological alterations that might take place during embryonic development.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A screening with different viral doses was conducted in embryos at HH Stage 10\u201312 (E1.5) as well as a follow up of the first 5\u2009days postinfection (dpi) was performed to observe the main morphologic changes post ZIKV infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>ZIKV exposed embryos presented a higher prevalence of mortality and defects such as brain malformation when compared to controls. Moreover, we observed that the phenotypes become more evident at 4dpi, when the viral load quantification reaches a peak.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We found that ZIKV exposed embryos presented a high prevalence of mortality and central nervous system (CNS) abnormalities in a dose\u2010dependent manner. The phenotype was more evident 4\u2009days postinfection, when the viral load quantification reached a peak.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdr2.1813","type":"journal-article","created":{"date-parts":[[2020,10,3]],"date-time":"2020-10-03T08:33:44Z","timestamp":1601714024000},"page":"22-31","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Zika virus\u2010induced brain malformations in chicken embryos"],"prefix":"10.1002","volume":"113","author":[{"given":"Gabriela Elis","family":"Wachholz","sequence":"first","affiliation":[{"name":"Postgraduate Program in Genetics and Molecular Biology, Department of Genetics Universidade Federal do Rio Grande do Sul  Porto Alegre Brazil"},{"name":"Teratogen Information Service Hospital de Cl\u00ednicas de Porto Alegre  Porto Alegre Brazil"},{"name":"Laboratory of Genomic Medicine, Experimental Research Center Hospital de Cl\u00ednicas de Porto Alegre  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula Muterle","family":"Varela","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biosciences Universidade Federal de Ci\u00eancias da Sa\u00fade de Porto Alegre  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thais Fumaco","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Parasitology Institute of Health Sciences, Universidade Federal do Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophia Martins Simon","family":"de Matos","sequence":"additional","affiliation":[{"name":"Laboratory of Genomic Medicine, Experimental Research Center Hospital de Cl\u00ednicas de Porto Alegre  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Rigon da Luz Soster","sequence":"additional","affiliation":[{"name":"Department of Morphological Sciences Institute of Health Sciences, Universidade Federal do Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda Sales Luiz","family":"Vianna","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Genetics and Molecular Biology, Department of Genetics Universidade Federal do Rio Grande do Sul  Porto Alegre Brazil"},{"name":"Teratogen Information Service Hospital de Cl\u00ednicas de Porto Alegre  Porto Alegre Brazil"},{"name":"Laboratory of Genomic Medicine, Experimental Research Center Hospital de Cl\u00ednicas de Porto Alegre  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diogo Onofre Gomes","family":"de Souza","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Biochemistry, Departamento f Biochemistry Institute of Health Sciences, Universidade Federal do Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo Michel","family":"Roehe","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Parasitology Institute of Health Sciences, Universidade Federal do Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lav\u00ednia","family":"Schuler\u2010Faccini","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Genetics and Molecular Biology, Department of Genetics Universidade Federal do Rio Grande do Sul  Porto Alegre Brazil"},{"name":"Teratogen Information Service Hospital de Cl\u00ednicas de Porto Alegre  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2555-8313","authenticated-orcid":false,"given":"Lucas Rosa","family":"Fraga","sequence":"additional","affiliation":[{"name":"Teratogen Information Service Hospital de Cl\u00ednicas de Porto Alegre  Porto Alegre Brazil"},{"name":"Laboratory of Genomic Medicine, Experimental Research Center Hospital de Cl\u00ednicas de Porto Alegre  Porto Alegre Brazil"},{"name":"Department of Morphological Sciences Institute of Health Sciences, Universidade Federal do Rio Grande do Sul  Porto Alegre Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,3]]},"reference":[{"issue":"7","key":"e_1_2_9_2_1","first-page":"36","article-title":"Image processing with ImageJ","volume":"11","author":"Abramoff M. 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