{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T13:09:51Z","timestamp":1770815391625,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T00:00:00Z","timestamp":1610668800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014337","name":"FURTHERMORE grants in publishing","doi-asserted-by":"publisher","award":["grant from Suzhou Municipality for science and tec"],"award-info":[{"award-number":["grant from Suzhou Municipality for science and tec"]}],"id":[{"id":"10.13039\/100014337","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014337","name":"FURTHERMORE grants in publishing","doi-asserted-by":"publisher","award":["grant from the Jiangsu Province for medical scienc"],"award-info":[{"award-number":["grant from the Jiangsu Province for medical scienc"]}],"id":[{"id":"10.13039\/100014337","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2021,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hypospadias is a common birth defect that might be caused by inadequate fusion of the urethral folds in the process of male external genital development. We intended to discover the crucial long noncoding RNAs (lncRNAs) regulating autophagy from the gene expression profile of the genital tubercle (GT) of dibutyl phthalate (DBP) induced hypospadiac rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Whole transcriptome resequencing was used to determine the expression of the total RNA in GTs and cultured fibroblasts obtained from GTs of DBP\u2010induced hypospadiac male rat fetuses. Autophagosomes and autolysosomes were examined under a transmission electron microscope after overexpression of lncRNA NONRATT008453.2 in the fibroblasts by adenovirus transfection. Finally, the protein expression levels of Atg5, Beclin\u20101, Atg7, and the LC3A\/B\u2010II:LC3A\/B\u2010I ratio were detected in the fibroblasts by western blotting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>NONRATT008453.2 suppressed autophagy by promoting the expression of Atg7, but inhibited the expressions of Atg5, Beclin\u20101, and the LC3A\/B\u2010II:LC3A\/B\u2010I ratio in the GT fibroblasts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>NONRATT008453.2 may have an influence on autophagy in the fibroblasts of the GT in DBP\u2010induced hypospadiac rats.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdr2.1863","type":"journal-article","created":{"date-parts":[[2021,1,16]],"date-time":"2021-01-16T06:47:45Z","timestamp":1610779665000},"page":"399-408","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The effects of <scp>NONRATT008453<\/scp>.2 on autophagy in genital tubercle fibroblasts of rats with hypospadias induced by dibutyl phthalate"],"prefix":"10.1002","volume":"113","author":[{"given":"Xiao","family":"Feng","sequence":"first","affiliation":[{"name":"Department of Clinical laboratory Children's Hospital of Soochow University  Suzhou P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enfu","family":"Huang","sequence":"additional","affiliation":[{"name":"Section of Pediatric Urology Children's Hospital of Soochow University  Suzhou P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanyuan","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Clinical laboratory Children's Hospital of Soochow University  Suzhou P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya","family":"Zhang","sequence":"additional","affiliation":[{"name":"Central Laboratory of Pediatric Research Institute Children's Hospital of Soochow University  Suzhou P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9374-5364","authenticated-orcid":false,"given":"Yun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Section of Pediatric Urology Children's Hospital of Soochow University  Suzhou P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)62711-3"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2011.08.019"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2012.06.003"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2013.00168"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.110.2.521"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-102808-114910"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2008.00899.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2010.191"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/15287390701432285"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12381"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1539\/joh.16-0089-OA"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.1995.tb07813.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.110544"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2008.51"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002840"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2017.5416"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2017.464"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2mb25386k"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2016.06.013"}],"container-title":["Birth Defects Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bdr2.1863","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/bdr2.1863","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/bdr2.1863","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T14:47:07Z","timestamp":1693666027000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/bdr2.1863"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,15]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,3,15]]}},"alternative-id":["10.1002\/bdr2.1863"],"URL":"https:\/\/doi.org\/10.1002\/bdr2.1863","archive":["Portico"],"relation":{},"ISSN":["2472-1727","2472-1727"],"issn-type":[{"value":"2472-1727","type":"print"},{"value":"2472-1727","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,15]]},"assertion":[{"value":"2020-02-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}