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Thalidomide is a drug responsible to a spectrum of congenital anomalies known as Thalidomide Embryopathy (TE), which includes mainly limb and heart defects. It is known that HAND2 interaction with TBX5, an important protein for limbs and heart development, is inhibited by Thalidomide. The aim of this study was to evaluate and characterize <jats:italic>HAND2<\/jats:italic> in the context of TE, and to evaluate its variability in TE individuals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>DNA from 35 TE subjects was extracted from saliva samples and PCR was performed for amplification and Sanger sequencing of <jats:italic>HAND2<\/jats:italic> coding sequence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The analysis showed only one variant; a synonymous variant p.P51 (rs59621536) in exon 1 found in three individuals. Further in silico evaluation confirmed highly <jats:italic>HAND2<\/jats:italic> conservation, being the 3\u2032UTR the most polymorphic region of the gene. Additional computational analyses classified the variant as neutral, without alteration in splicing and miRNA sites. In silico predictions pointed to alteration of two CpG islands adjacent to the variant; however, we did not observe any alterations on the methylation pattern of <jats:italic>HAND2<\/jats:italic> gene in our sample. Moreover, alteration of the binding site of MeCP2, a nuclear protein involved in DNA methylation, was predicted along with alteration in <jats:italic>HAND2<\/jats:italic> mRNA structure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Considering <jats:italic>HAND2<\/jats:italic> being a well conserved gene, further studies with a larger sample should be performed to evaluate the role this gene on genetic susceptibility to TE.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdr2.2092","type":"journal-article","created":{"date-parts":[[2022,9,30]],"date-time":"2022-09-30T09:10:51Z","timestamp":1664529051000},"page":"1354-1363","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genetic evaluation of <i>HAND2<\/i> gene and its effects on thalidomide embryopathy"],"prefix":"10.1002","volume":"114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4100-8629","authenticated-orcid":false,"given":"Bruna 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