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To investigate and summarize how to isolate and culture the CNCCs from wild\u2010type mice, a literature search was performed in online databases (PubMed and Web of Science) using optimized keywords \u201cmouse,\u201d \u201ccranial neural crest cell\u201d and \u201cculture.\u201d The literature was checked by two investigators according to the screening and exclusion criteria. Initially, 197 studies were retrieved from PubMed and 169 from Web of Science, and after excluding replicate studies, 293 articles were considered. Finally, 17 studies met all the criteria and were included in this review. The results showed that obtaining purified stem cells and balancing the need to promote cell growth and prevent unwanted early cell differentiation were the two key points in the isolation and culture of CNCCs. However, no standard criteria are available for answering these questions. 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