{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T16:20:41Z","timestamp":1792858841807,"version":"4.5.0"},"reference-count":33,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T00:00:00Z","timestamp":1696032000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81901485"],"award-info":[{"award-number":["81901485"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82170606"],"award-info":[{"award-number":["82170606"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Birth Defects Research"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Maternal diabetes increases the risk for neural tube defects (NTDs). It is unclear if miRNAs, senescence, and DNA damage are involved in this process. In this study, we used neural stem cells as an in vitro proxy of embryonic neuroepithelium to investigate whether high glucose triggers neural stem cell senescence and DNA damage by upregulating miR\u2010200c, which may be responsible for NTDs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>C17.2 neural stem cells were cultured with normal glucose (5\u2009mM) or high glucose (\u226516.7\u2009mM) at different doses and time points for detecting miR\u2010200c levels, markers of senescence and DNA damage. Neural stem cells were exposed to antioxidant SOD1 mimetic Tempol and high glucose for 48\u2009h to test roles of oxidative stress on the miR\u2010200c, senescence, and DNA damage levels. An miR\u2010200c mimic and an inhibitor were transfected into neural stem cells to increase or decrease miR\u2010200c activities.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>High glucose upregulated miR\u2010200c in neural stem cells. A time course study of the effect of high glucose revealed that miR\u2010200c initially increased at 12\u2009h and reached its zenith at 18\u2009h. Tempol reduced miR\u2010200c levels caused by high glucose. High glucose induced markers of senescence and DNA damage in neural stem cells. Tempol abolished high glucose\u2010induced markers of senescence and DNA damage. The miR\u2010200c inhibitor suppressed high glucose\u2010induced markers of senescence and DNA damage. Treatment with miR\u2010200c mimic imitates high glucose\u2010induced markers of senescence and DNA damage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We show that high glucose increases miR\u2010200c, which contributes to cellular senescence and DNA damage in neural stem cells and provides a potential pathway for maternal diabetes\u2010induced neural tube defects.<\/jats:p><\/jats:sec>","DOI":"10.1002\/bdr2.2254","type":"journal-article","created":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T19:34:46Z","timestamp":1696102486000},"page":"1770-1779","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["High glucose\u2010increased <scp>miR<\/scp>\u2010200c contributes to cellular senescence and <scp>DNA<\/scp> damage in neural stem cells"],"prefix":"10.1002","volume":"115","author":[{"given":"Dao\u2010Yin","family":"Dong","sequence":"first","affiliation":[{"name":"Department of Public Health, School of Basic Medical Sciences Henan University of Science and Technology  Luoyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pu\u2010Yu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of General Medicine The First Affiliated Hospital of Henan University of Science and Technology  Luoyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying\u2010Fang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Public Health, School of Basic Medical Sciences Henan University of Science and Technology  Luoyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Public Health, School of Basic Medical Sciences Henan University of Science and Technology  Luoyang 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